The onset of foreign gene transcription in nuclear-transferred embryos of fish
文献类型:期刊论文
作者 | Sun, YH; Chen, SP; Wang, YP; Zhu, ZY |
刊名 | SCIENCE IN CHINA SERIES C-LIFE SCIENCES |
出版日期 | 2000-12-01 |
卷号 | 43期号:6页码:597-605 |
ISSN号 | 1006-9305 |
关键词 | transgenic fish nuclear transplantation serial nuclear transplantation transcription reverse transcriptional PCR reprogramming |
通讯作者 | Zhu, ZY, Wuhan Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
中文摘要 | The transcriptional onset of hGH-transgene in fish was studied in the following three cases: the first is in MThGH-transgenic F-4 common carp (Cyprinus carpio) embryos, the second is in nuclear-transferred embryos supported by the transgenic F-4 embryonic nuclei, and the third is in nuclear-transferred embryos supported by the transgenic F-4 tail-fin nuclei. RT-PCR results show that the hGH-transgene initiates its transcriptional activity from early-gastrula stage, the early blastula stage and even 16-cell stage in the first, second and third cases, respectively. it looks like that fish egg cytoplasm could just offer a very restricted reprogramming on transcriptional activity of specific gene in differentiated cell nuclei by nuclear transplantation. |
英文摘要 | The transcriptional onset of hGH-transgene in fish was studied in the following three cases: the first is in MThGH-transgenic F-4 common carp (Cyprinus carpio) embryos, the second is in nuclear-transferred embryos supported by the transgenic F-4 embryonic nuclei, and the third is in nuclear-transferred embryos supported by the transgenic F-4 tail-fin nuclei. RT-PCR results show that the hGH-transgene initiates its transcriptional activity from early-gastrula stage, the early blastula stage and even 16-cell stage in the first, second and third cases, respectively. it looks like that fish egg cytoplasm could just offer a very restricted reprogramming on transcriptional activity of specific gene in differentiated cell nuclei by nuclear transplantation. |
学科主题 | Biology |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Biology |
研究领域[WOS] | Life Sciences & Biomedicine - Other Topics |
关键词[WOS] | NUCLEOLAR ULTRASTRUCTURE ; BOVINE EMBRYO ; TRANSPLANTATION ; MATURATION ; MAMMALS |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000166396000005 |
公开日期 | 2010-10-13 |
源URL | [http://ir.ihb.ac.cn/handle/152342/10046] |
专题 | 水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文 |
作者单位 | Wuhan Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
推荐引用方式 GB/T 7714 | Sun, YH,Chen, SP,Wang, YP,et al. The onset of foreign gene transcription in nuclear-transferred embryos of fish[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2000,43(6):597-605. |
APA | Sun, YH,Chen, SP,Wang, YP,&Zhu, ZY.(2000).The onset of foreign gene transcription in nuclear-transferred embryos of fish.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,43(6),597-605. |
MLA | Sun, YH,et al."The onset of foreign gene transcription in nuclear-transferred embryos of fish".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 43.6(2000):597-605. |
入库方式: OAI收割
来源:水生生物研究所
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