The onset of foreign gene transcription in nuclear-transferred embryos of fish
文献类型：期刊论文
| 作者 | Sun, YH; Chen, SP; Wang, YP; Zhu, ZY |
| 刊名 | SCIENCE IN CHINA SERIES C-LIFE SCIENCES
 |
| 出版日期 | 2000-12-01 |
| 卷号 | 43期号:6页码:597-605 |
| 关键词 | transgenic fish nuclear transplantation serial nuclear transplantation transcription reverse transcriptional PCR reprogramming |
| ISSN号 | 1006-9305 |
| 通讯作者 | Zhu, ZY, Wuhan Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | The transcriptional onset of hGH-transgene in fish was studied in the following three cases: the first is in MThGH-transgenic F-4 common carp (Cyprinus carpio) embryos, the second is in nuclear-transferred embryos supported by the transgenic F-4 embryonic nuclei, and the third is in nuclear-transferred embryos supported by the transgenic F-4 tail-fin nuclei. RT-PCR results show that the hGH-transgene initiates its transcriptional activity from early-gastrula stage, the early blastula stage and even 16-cell stage in the first, second and third cases, respectively. it looks like that fish egg cytoplasm could just offer a very restricted reprogramming on transcriptional activity of specific gene in differentiated cell nuclei by nuclear transplantation. |
| 英文摘要 | The transcriptional onset of hGH-transgene in fish was studied in the following three cases: the first is in MThGH-transgenic F-4 common carp (Cyprinus carpio) embryos, the second is in nuclear-transferred embryos supported by the transgenic F-4 embryonic nuclei, and the third is in nuclear-transferred embryos supported by the transgenic F-4 tail-fin nuclei. RT-PCR results show that the hGH-transgene initiates its transcriptional activity from early-gastrula stage, the early blastula stage and even 16-cell stage in the first, second and third cases, respectively. it looks like that fish egg cytoplasm could just offer a very restricted reprogramming on transcriptional activity of specific gene in differentiated cell nuclei by nuclear transplantation. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biology |
| 类目[WOS] | Biology |
| 研究领域[WOS] | Life Sciences & Biomedicine - Other Topics |
| 关键词[WOS] | NUCLEOLAR ULTRASTRUCTURE ; BOVINE EMBRYO ; TRANSPLANTATION ; MATURATION ; MAMMALS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000166396000005 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/10046]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Wuhan Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, YH,Chen, SP,Wang, YP,et al. The onset of foreign gene transcription in nuclear-transferred embryos of fish[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2000,43(6):597-605. |
| APA | Sun, YH,Chen, SP,Wang, YP,&Zhu, ZY.(2000).The onset of foreign gene transcription in nuclear-transferred embryos of fish.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,43(6),597-605. |
| MLA | Sun, YH,et al."The onset of foreign gene transcription in nuclear-transferred embryos of fish".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 43.6(2000):597-605. |
入库方式： OAI收割
来源：水生生物研究所
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