RAPD and AFLP techniques for the analysis of genetic relationships in two genera of Decapoda
文献类型:期刊论文
作者 | Lu, RH; Qiu, T; Xiang, CM; Xie, H; Zhang, J |
刊名 | CRUSTACEANA
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出版日期 | 2000-11-01 |
卷号 | 73期号:0页码:1027-1036 |
关键词 | ARBITRARY PRIMERS MITTEN CRAB MARKERS ERIOCHEIR DNA PCR |
ISSN号 | 0011-216X |
通讯作者 | Lu, RH, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
中文摘要 | RAPD was used fur analysing three (sub-)species of mitten crabs (Eriocheir sinensis, E. japonicus, and E. japonicus hepuensis) and three populations of E. sinensis. The results show that their relationships on DNA level are similar to the classical taxonomic hypotheses (Dai, 1991). No diagnostic RAPD marker could be found, but there were statistically significant genetic differences among these taxa (P < 0.001) or populations (P < 0.001). That is, the intraspecific similarities were larger than the interspecific similarities; the intrasubspecific similarities were larger than the intraspecific similarities; and the intrapopulational similarities were larger than the interpopulational similarities. In AFLP analysis, no significant genetic difference has been found between E. sinensis and E. japonicus, but AFLP markers among four species of Macrobrachium (M. rosenbergii. M. nipponense, M. hainanense, and M. asperulum) were found. The DNA similarities among these four species of Macrobrachium are in accordance with morphological similarities. |
英文摘要 | RAPD was used fur analysing three (sub-)species of mitten crabs (Eriocheir sinensis, E. japonicus, and E. japonicus hepuensis) and three populations of E. sinensis. The results show that their relationships on DNA level are similar to the classical taxonomic hypotheses (Dai, 1991). No diagnostic RAPD marker could be found, but there were statistically significant genetic differences among these taxa (P < 0.001) or populations (P < 0.001). That is, the intraspecific similarities were larger than the interspecific similarities; the intrasubspecific similarities were larger than the intraspecific similarities; and the intrapopulational similarities were larger than the interpopulational similarities. In AFLP analysis, no significant genetic difference has been found between E. sinensis and E. japonicus, but AFLP markers among four species of Macrobrachium (M. rosenbergii. M. nipponense, M. hainanense, and M. asperulum) were found. The DNA similarities among these four species of Macrobrachium are in accordance with morphological similarities. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
学科主题 | Marine & Freshwater Biology |
类目[WOS] | Marine & Freshwater Biology |
研究领域[WOS] | Marine & Freshwater Biology |
关键词[WOS] | ARBITRARY PRIMERS ; MITTEN CRAB ; MARKERS ; ERIOCHEIR ; DNA ; PCR |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000166405700001 |
公开日期 | 2010-10-13 |
源URL | [http://ir.ihb.ac.cn/handle/152342/10050] ![]() |
专题 | 水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文 |
作者单位 | Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
推荐引用方式 GB/T 7714 | Lu, RH,Qiu, T,Xiang, CM,et al. RAPD and AFLP techniques for the analysis of genetic relationships in two genera of Decapoda[J]. CRUSTACEANA,2000,73(0):1027-1036. |
APA | Lu, RH,Qiu, T,Xiang, CM,Xie, H,&Zhang, J.(2000).RAPD and AFLP techniques for the analysis of genetic relationships in two genera of Decapoda.CRUSTACEANA,73(0),1027-1036. |
MLA | Lu, RH,et al."RAPD and AFLP techniques for the analysis of genetic relationships in two genera of Decapoda".CRUSTACEANA 73.0(2000):1027-1036. |
入库方式: OAI收割
来源:水生生物研究所
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