Nuclear transplantation with early-embryonic cells of transgenic fish
文献类型：期刊论文
| 作者 | Chen, SP; Zhao, HB; Sun, YH; Zhang, FY; Zhu, ZY |
| 刊名 | PROGRESS IN NATURAL SCIENCE
 |
| 出版日期 | 2000-12-01 |
| 卷号 | 10期号:12页码:925-930 |
| 关键词 | fish gene transfer nuclear transplantation |
| ISSN号 | 1002-0071 |
| 通讯作者 | Chen, SP, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Like other transgenic animals, transgenic fishes produced by microinjection are transgenic mosaics. In order to produce homogenous transgenic fish, the transgenic blastula or gastrula cells were dissociated from Carassius auratus, Pengze var, and Cyprinus carpio, Huanghe var., and the nuclei were transferred into the mature eggs of the same species via microinjection or electro-fusion. Five nuclear-transferred Carassius auratus, Pengze var. and one Cyprinus carpio, Huanghe var. were obtained and the existence of the transgene was detected. The possibility of generating homogenous strain of transgenic fish by nuclear transplantation with transgenic early-embryonic cells is discussed. |
| 英文摘要 | Like other transgenic animals, transgenic fishes produced by microinjection are transgenic mosaics. In order to produce homogenous transgenic fish, the transgenic blastula or gastrula cells were dissociated from Carassius auratus, Pengze var, and Cyprinus carpio, Huanghe var., and the nuclei were transferred into the mature eggs of the same species via microinjection or electro-fusion. Five nuclear-transferred Carassius auratus, Pengze var. and one Cyprinus carpio, Huanghe var. were obtained and the existence of the transgene was detected. The possibility of generating homogenous strain of transgenic fish by nuclear transplantation with transgenic early-embryonic cells is discussed. |
| WOS标题词 | Science & Technology ; Technology |
| 学科主题 | Multidisciplinary Sciences |
| 类目[WOS] | Materials Science, Multidisciplinary ; Multidisciplinary Sciences |
| 研究领域[WOS] | Materials Science ; Science & Technology - Other Topics |
| 关键词[WOS] | ORYZIAS-LATIPES ; ESTABLISHMENT ; CULTURES ; GROWTH ; MEDAKA ; EGGS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000165659900006 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/10056]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, SP,Zhao, HB,Sun, YH,et al. Nuclear transplantation with early-embryonic cells of transgenic fish[J]. PROGRESS IN NATURAL SCIENCE,2000,10(12):925-930. |
| APA | Chen, SP,Zhao, HB,Sun, YH,Zhang, FY,&Zhu, ZY.(2000).Nuclear transplantation with early-embryonic cells of transgenic fish.PROGRESS IN NATURAL SCIENCE,10(12),925-930. |
| MLA | Chen, SP,et al."Nuclear transplantation with early-embryonic cells of transgenic fish".PROGRESS IN NATURAL SCIENCE 10.12(2000):925-930. |
入库方式： OAI收割
来源：水生生物研究所
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