Studies of polysaccharides from three edible species of Nostoc (cyanobacteria) with different colony morphologies: Structural characterization and effect on the complement system of polysaccharides from Nostoc commune
文献类型：期刊论文
| 作者 | Brull, LP; Huang, ZB; Thomas-Oates, JE; Paulsen, BS; Cohen, EH; Michaelsen, TE |
| 刊名 | JOURNAL OF PHYCOLOGY
 |
| 出版日期 | 2000-10-01 |
| 卷号 | 36期号:5页码:871-881 |
| ISSN号 | 0022-3646 |
| 关键词 | complement activity Cyanobacteria HPAEC 2-O-methylglucose Nostoc commune oligosaccharide profiles polysaccharides |
| 通讯作者 | Paulsen, BS, Univ Oslo, Sch Pharm, Dept Pharmacognosy, POB 1068, N-0316 Oslo, Norway |
| 中文摘要 | The cyanobacterium Nostoc commune Vaucher produces quite complex extracellular polysaccharides. The cyanobacterium is nitrogen fixing, and on growing the cyanobacterium in media with and without nitrogen, different types of extracellular polysaccharides were obtained. These were also different from the polysaccharides present in N. commune collected in the field. High pH anion exchange chromatography (HPAEC) of weak acid hydrolysates of the culture-grown material demonstrated that, in this case, HPAEC was useful for comparison of the different polymers. The main differences between the polymers from the field group and the culture-grown samples were the presence of substantial amounts of arabinose, 2-O-methylglucose, and glucuronic acid in the latter. Methylation studies also revealed a difference in the branching points on the glucose units between the field and cultured samples, being 1,4,6 for the first and 1,3,6 for the latter. The field acidic fraction gave, on weak acid hydrolysis and separation on BioGel P2 and HPAEC, 12 oligosaccharide fractions that were isolated and studied by different mass spectroscopy techniques. The structures of the oligosaccharides were determined, and two different series that can originate from two repeating pentamers were identified: GlcAl-4/6GlcM1-4Ga11-4Glc1-4Xyl and GlcAl-4/6Glc1-4Ga11-4Glc1-4Xyl. The difference between these oligosaccharides lies in the methyl substituent on carbon 2 of the glucose unit next to the nonreducing glucuronic acid unit. The polysaccharides from field material were shown to have a strong effect on the complement system. |
| 英文摘要 | The cyanobacterium Nostoc commune Vaucher produces quite complex extracellular polysaccharides. The cyanobacterium is nitrogen fixing, and on growing the cyanobacterium in media with and without nitrogen, different types of extracellular polysaccharides were obtained. These were also different from the polysaccharides present in N. commune collected in the field. High pH anion exchange chromatography (HPAEC) of weak acid hydrolysates of the culture-grown material demonstrated that, in this case, HPAEC was useful for comparison of the different polymers. The main differences between the polymers from the field group and the culture-grown samples were the presence of substantial amounts of arabinose, 2-O-methylglucose, and glucuronic acid in the latter. Methylation studies also revealed a difference in the branching points on the glucose units between the field and cultured samples, being 1,4,6 for the first and 1,3,6 for the latter. The field acidic fraction gave, on weak acid hydrolysis and separation on BioGel P2 and HPAEC, 12 oligosaccharide fractions that were isolated and studied by different mass spectroscopy techniques. The structures of the oligosaccharides were determined, and two different series that can originate from two repeating pentamers were identified: GlcAl-4/6GlcM1-4Ga11-4Glc1-4Xyl and GlcAl-4/6Glc1-4Ga11-4Glc1-4Xyl. The difference between these oligosaccharides lies in the methyl substituent on carbon 2 of the glucose unit next to the nonreducing glucuronic acid unit. The polysaccharides from field material were shown to have a strong effect on the complement system. |
| 学科主题 | Plant Sciences; Marine & Freshwater Biology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Plant Sciences ; Marine & Freshwater Biology |
| 研究领域[WOS] | Plant Sciences ; Marine & Freshwater Biology |
| 关键词[WOS] | MASS-SPECTROMETRY ; METHYLATION ANALYSIS ; OLIGOSACCHARIDES ; CELL ; SEPARATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000165409200011 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/10058]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Univ Oslo, Sch Pharm, Dept Pharmacognosy, N-0316 Oslo, Norway 2.TNO, Netherlands Org Appl Sci Res, NL-3700 AJ Zeist, Netherlands 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 4.Univ Manchester, Inst Sci & Technol, Dept Chem, Michael Barber Ctr Mass Spectrometry, Manchester M60 1QD, Lancs, England 5.Natl Inst Publ Hlth, Dept Vaccinol, N-0403 Oslo, Norway |
| 推荐引用方式 GB/T 7714 | Brull, LP,Huang, ZB,Thomas-Oates, JE,et al. Studies of polysaccharides from three edible species of Nostoc (cyanobacteria) with different colony morphologies: Structural characterization and effect on the complement system of polysaccharides from Nostoc commune[J]. JOURNAL OF PHYCOLOGY,2000,36(5):871-881. |
| APA | Brull, LP,Huang, ZB,Thomas-Oates, JE,Paulsen, BS,Cohen, EH,&Michaelsen, TE.(2000).Studies of polysaccharides from three edible species of Nostoc (cyanobacteria) with different colony morphologies: Structural characterization and effect on the complement system of polysaccharides from Nostoc commune.JOURNAL OF PHYCOLOGY,36(5),871-881. |
| MLA | Brull, LP,et al."Studies of polysaccharides from three edible species of Nostoc (cyanobacteria) with different colony morphologies: Structural characterization and effect on the complement system of polysaccharides from Nostoc commune".JOURNAL OF PHYCOLOGY 36.5(2000):871-881. |
入库方式： OAI收割
来源：水生生物研究所
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