Analysis of expression of the binary toxin genes from Bacillus sphaericus in Anabaena and the potential in mosquito control
文献类型：期刊论文
| 作者 | Xu, XD; Yan, G; Kong, RQ; Liu, XP; Yu, LH |
| 刊名 | CURRENT MICROBIOLOGY
 |
| 出版日期 | 2000-11-01 |
| 卷号 | 41期号:5页码:352-356 |
| 关键词 | THURINGIENSIS SUBSP ISRAELENSIS |
| ISSN号 | 0343-8651 |
| 通讯作者 | Xu, XD, Shandong Inst Parasit Dis, Shandong 272133, Peoples R China |
| 中文摘要 | Anabaena strains expressing the binary toxin genes of Bacillus sphaericus produce high larvicidal activity with living cells. Western blot analysis showed that the 51-kDa and 42-kDa toxin proteins were stable in Anabaena. When a DNA fragment upstream of the 51-kDa protein gene was deleted, the toxicity was reduced by over a hundred-fold, whereas deletions at the coding regions showed that the cooperation of the two proteins expressed in Anabaena is essential for the larvicidal activity. Outdoor tests showed that the genetically altered Anabaena could keep containers with natural water from being inhabited by Culex larvae for over 2 months. |
| 英文摘要 | Anabaena strains expressing the binary toxin genes of Bacillus sphaericus produce high larvicidal activity with living cells. Western blot analysis showed that the 51-kDa and 42-kDa toxin proteins were stable in Anabaena. When a DNA fragment upstream of the 51-kDa protein gene was deleted, the toxicity was reduced by over a hundred-fold, whereas deletions at the coding regions showed that the cooperation of the two proteins expressed in Anabaena is essential for the larvicidal activity. Outdoor tests showed that the genetically altered Anabaena could keep containers with natural water from being inhabited by Culex larvae for over 2 months. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Microbiology |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | THURINGIENSIS SUBSP ISRAELENSIS ; AGMENELLUM-QUADRUPLICATUM PR-6 ; LARVICIDAL ACTIVITY ; STRAIN PCC-7120 ; CYANOBACTERIUM ; PROTEINS ; CLONING ; LARVAE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000089989600009 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/10078]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Shandong Inst Parasit Dis, Shandong 272133, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, XD,Yan, G,Kong, RQ,et al. Analysis of expression of the binary toxin genes from Bacillus sphaericus in Anabaena and the potential in mosquito control[J]. CURRENT MICROBIOLOGY,2000,41(5):352-356. |
| APA | Xu, XD,Yan, G,Kong, RQ,Liu, XP,&Yu, LH.(2000).Analysis of expression of the binary toxin genes from Bacillus sphaericus in Anabaena and the potential in mosquito control.CURRENT MICROBIOLOGY,41(5),352-356. |
| MLA | Xu, XD,et al."Analysis of expression of the binary toxin genes from Bacillus sphaericus in Anabaena and the potential in mosquito control".CURRENT MICROBIOLOGY 41.5(2000):352-356. |
入库方式： OAI收割
来源：水生生物研究所
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