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Effects of heavy metals on the expression of a zinc-inducible metallothionein-III gene and antioxidant enzyme activities in Crassostrea gigas

文献类型：期刊论文

作者Cong, Ming1; Wu, Huifeng1; Liu, Xiaoli1,2; Zhao, Jianmin1; Wang, Xuan3; Lv, Jiasen4; Hou, Lin4
刊名ECOTOXICOLOGY
出版日期2012-10-01
卷号21期号:7页码:1928-1936
关键词Crassostrea gigas Metallothionein Cadmium Zinc Antioxidant enzyme
ISSN号0963-9292
产权排序[Cong, Ming; Wu, Huifeng; Liu, Xiaoli; Zhao, Jianmin] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China; [Liu, Xiaoli] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Wang, Xuan] Ocean Univ China, Qingdao 266100, Shandong, Peoples R China; [Lv, Jiasen; Hou, Lin] Yantai Univ, Ocean Sch, Yantai 264005, Shandong, Peoples R China
通讯作者Wu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China.
中文摘要Sequestration by metallothioneins and antioxidant defense are two kinds of important defense mechanisms employed by mollusks to minimize adverse effects caused by heavy metal contaminants in marine environment. In the present study, a novel metallothionein gene, CgMT-III, was cloned from Crassostrea gigas, consisting of eighteen conserved cysteine residues and encoding a MT III-like protein with two tandem beta domains. The expression level of CgMT-III transcript induced by zinc was much higher than that induced by cadmium exposure. It suggested that CgMT-III was perhaps mainly involved in homeostatic control of zinc metabolism, which was distinct from previously identified MTs in C. gigas. Among the tested antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), SOD and GPx showed varying up-regulations in a tissue-specific manner, while CAT activities were inhibited in both gill and hepatopancreas from C. gigas exposed to heavy metals. It can be inferred that CgMT-III was mainly involved in zinc homeostasis, and CgMT-III gene together with CAT enzyme could be potential biomarkers to indicate heavy metal, especially zinc pollution in marine organisms.
英文摘要Sequestration by metallothioneins and antioxidant defense are two kinds of important defense mechanisms employed by mollusks to minimize adverse effects caused by heavy metal contaminants in marine environment. In the present study, a novel metallothionein gene, CgMT-III, was cloned from Crassostrea gigas, consisting of eighteen conserved cysteine residues and encoding a MT III-like protein with two tandem beta domains. The expression level of CgMT-III transcript induced by zinc was much higher than that induced by cadmium exposure. It suggested that CgMT-III was perhaps mainly involved in homeostatic control of zinc metabolism, which was distinct from previously identified MTs in C. gigas. Among the tested antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), SOD and GPx showed varying up-regulations in a tissue-specific manner, while CAT activities were inhibited in both gill and hepatopancreas from C. gigas exposed to heavy metals. It can be inferred that CgMT-III was mainly involved in zinc homeostasis, and CgMT-III gene together with CAT enzyme could be potential biomarkers to indicate heavy metal, especially zinc pollution in marine organisms.
学科主题Environmental Sciences & Ecology; Toxicology
研究领域[WOS]Environmental Sciences & Ecology ; Toxicology
关键词[WOS]MESSENGER-RNA EXPRESSION ; PACIFIC OYSTER ; CADMIUM EXPOSURE ; RUDITAPES-DECUSSATUS ; SUPEROXIDE DISMUTASE ; LIPID-PEROXIDATION ; VIRGINICA GMELIN ; STRESS-PROTEINS ; IN-VITRO ; COPPER
收录类别SCI
资助信息Chinese Academy of Sciences; NSFC [31172388]; SDSFC [ZR2009CZ008]
原文出处http://dx.doi.org/10.1007/s10646-012-0926-z
语种英语
WOS记录号WOS:000308235500016
公开日期2013-03-08
源URL[http://ir.yic.ac.cn/handle/133337/5985]  
专题烟台海岸带研究所_污染过程与控制实验室
烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Ocean Univ China, Qingdao 266100, Shandong, Peoples R China
4.Yantai Univ, Ocean Sch, Yantai 264005, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Cong, Ming,Wu, Huifeng,Liu, Xiaoli,et al. Effects of heavy metals on the expression of a zinc-inducible metallothionein-III gene and antioxidant enzyme activities in Crassostrea gigas[J]. ECOTOXICOLOGY,2012,21(7):1928-1936.
APA Cong, Ming.,Wu, Huifeng.,Liu, Xiaoli.,Zhao, Jianmin.,Wang, Xuan.,...&Hou, Lin.(2012).Effects of heavy metals on the expression of a zinc-inducible metallothionein-III gene and antioxidant enzyme activities in Crassostrea gigas.ECOTOXICOLOGY,21(7),1928-1936.
MLA Cong, Ming,et al."Effects of heavy metals on the expression of a zinc-inducible metallothionein-III gene and antioxidant enzyme activities in Crassostrea gigas".ECOTOXICOLOGY 21.7(2012):1928-1936.

入库方式： OAI收割

来源：烟台海岸带研究所

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