Proteomic response of wheat embryos to fosthiazate stress in a protected vegetable soil
文献类型：期刊论文
| 作者 | Yin, Chunyan1,2; Teng, Ying1,2; Luo, Yongming1,2,3 ; Christie, Peter4
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| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
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| 出版日期 | 2012 |
| 卷号 | 24期号:10页码:1843-1853 |
| 关键词 | fosthiazate proteomic wheat embryo stress response biological indicator |
| ISSN号 | 1001-0742 |
| 产权排序 | [Yin, Chunyan; Teng, Ying; Luo, Yongming] Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China; [Yin, Chunyan; Teng, Ying; Luo, Yongming] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Luo, Yongming] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Christie, Peter] Agri Food & Biosci Inst, Agri Environm Branch, Belfast BT9 5PX, Antrim, North Ireland |
| 通讯作者 | Luo, YM (reprint author), Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China.,cyyin@issas.ac.cn ; ymluo@yic.ac.cn |
| 中文摘要 | A proteomic analysis of wheat defense response induced by the widely used organophosphorus nematicide fosthiazate is reported. Seed germination and two-dimensional gel electrophoresis (2-DE) experiments were performed using a Chinese wheat cultivar, Zhenmai No. 5. Root and shoot elongation decreased but thiobarbituric acid reactive substances (TBARS) content in embryos increased with increasing pesticide concentration. More than 1000 protein spots were reproducibly detected in each silver-stained gel. Thirty-seven protein spots with at least 2-fold changes were identified using MALDI-TOF MS/MS analysis. Of these, 24 spots were up-regulated and 13 were down-regulated. Proteins identified included some well-known classical stress responsive proteins under abiotic or biotic stresses as well as some unusual responsive proteins. Ten responsive proteins were reported for the first time at the proteomic level, including fatty acyl CoA reductase, dihydrodipicolinate synthase, DEAD-box ATPase-RNA-helicase, fimbriata-like protein, waxy B1, rust resistance kinase Lr10, putative 12.1 protein, retinoblastoma-related protein 1, pollen allergen-like protein and S-adenosyl-L-methionine:phosphoethanolamine N-methyltransferase. The proteins identified were involved in several processes such as metabolism, defense/detoxification, cell structure/cell growth, signal transduction/transcription, photosynthesis and energy. Seven candidate proteins were further analyzed at the mRNA level by RT-PCR to compare transcript and protein accumulation patterns, revealing that not all the genes were correlated well with the protein level. Identification of these responsive proteins may provide new insight into the molecular basis of the fosthiazate-stress response in the early developmental stages of plants and may be useful in stress monitoring or stress-tolerant crop breeding for environmentally friendly agricultural production. |
| 英文摘要 | A proteomic analysis of wheat defense response induced by the widely used organophosphorus nematicide fosthiazate is reported. Seed germination and two-dimensional gel electrophoresis (2-DE) experiments were performed using a Chinese wheat cultivar, Zhenmai No. 5. Root and shoot elongation decreased but thiobarbituric acid reactive substances (TBARS) content in embryos increased with increasing pesticide concentration. More than 1000 protein spots were reproducibly detected in each silver-stained gel. Thirty-seven protein spots with at least 2-fold changes were identified using MALDI-TOF MS/MS analysis. Of these, 24 spots were up-regulated and 13 were down-regulated. Proteins identified included some well-known classical stress responsive proteins under abiotic or biotic stresses as well as some unusual responsive proteins. Ten responsive proteins were reported for the first time at the proteomic level, including fatty acyl CoA reductase, dihydrodipicolinate synthase, DEAD-box ATPase-RNA-helicase, fimbriata-like protein, waxy B1, rust resistance kinase Lr10, putative 12.1 protein, retinoblastoma-related protein 1, pollen allergen-like protein and S-adenosyl-L-methionine:phosphoethanolamine N-methyltransferase. The proteins identified were involved in several processes such as metabolism, defense/detoxification, cell structure/cell growth, signal transduction/transcription, photosynthesis and energy. Seven candidate proteins were further analyzed at the mRNA level by RT-PCR to compare transcript and protein accumulation patterns, revealing that not all the genes were correlated well with the protein level. Identification of these responsive proteins may provide new insight into the molecular basis of the fosthiazate-stress response in the early developmental stages of plants and may be useful in stress monitoring or stress-tolerant crop breeding for environmentally friendly agricultural production. |
| 学科主题 | Environmental Sciences |
| 研究领域[WOS] | Environmental Sciences & Ecology |
| 关键词[WOS] | NON-FUMIGANT NEMATICIDES ; HEAT-SHOCK PROTEINS ; TRITICUM-AESTIVUM ; OXIDATIVE STRESS ; N-METHYLTRANSFERASE ; TUBULIN GENES ; CELL-DIVISION ; SALT STRESS ; RICE ; PLANTS |
| 收录类别 | SCI |
| 资助信息 | National Environmental Protection special funds for scientific research on public causes of China [2010467016]; Natural Science Foundation of Jiangsu Province [BK2009016]; Chinese Academy of Sciences [KZCX2-YW-Q02-06-02, KZCW2-YW-404] |
| 原文出处 | http://dx.doi.org/10.1016/S1001-0742(11)61013-9 |
| 语种 | 英语 |
| WOS记录号 | WOS:000310408700016 |
| 公开日期 | 2013-03-08 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/6039]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 4.Agri Food & Biosci Inst, Agri Environm Branch, Belfast BT9 5PX, Antrim, North Ireland |
| 推荐引用方式 GB/T 7714 | Yin, Chunyan,Teng, Ying,Luo, Yongming,et al. Proteomic response of wheat embryos to fosthiazate stress in a protected vegetable soil[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2012,24(10):1843-1853. |
| APA | Yin, Chunyan,Teng, Ying,Luo, Yongming,&Christie, Peter.(2012).Proteomic response of wheat embryos to fosthiazate stress in a protected vegetable soil.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,24(10),1843-1853. |
| MLA | Yin, Chunyan,et al."Proteomic response of wheat embryos to fosthiazate stress in a protected vegetable soil".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 24.10(2012):1843-1853. |
入库方式： OAI收割
来源：烟台海岸带研究所
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