中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Sensitive Near-Infrared Fluorescent Probes for Thiols Based on Se-N Bond Cleavage: Imaging in Living Cells and Tissues

文献类型:期刊论文

作者Wang, Rui; Chen, Lingxin; Liu, Ping; Zhang, Qin; Wang, Yunqing
刊名CHEMISTRY-A EUROPEAN JOURNAL
出版日期2012-09-01
卷号18期号:36页码:11343-11349
关键词cleavage reactions fluorescent probes imaging agents Se-N bonds thiols
ISSN号0947-6539
产权排序[Wang, Rui; Chen, Lingxin; Liu, Ping; Zhang, Qin; Wang, Yunqing] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proce, Yantai 264003, Shandong, Peoples R China
通讯作者Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proce, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R China.,lxchen@yic.ac.cn
中文摘要Cy-NiSe and Cy-TfSe were designed and synthesized as sensitive near-infrared (NIR) fluorescent probes for detecting thiols on the basis of Se-N bond cleavage both in cells and in tissues. Since a donor-excited photoinduced electron transfer (d-PET) process occurs between the modulator and the fluorophore, Cy-NiSe and Cy-TfSe have weak fluorescence. On titration with glutathione, the free dye exhibits significant fluorescence enhancement. The two probes are sensitive and selective for thiols over other relevant biological species. They can function rapidly at pH 7.4, and their emission lies in the NIR region. Confocal imaging confirms that Cy-NiSe and Cy-TfSe can be used for detecting thiols in living cells and tissues.
英文摘要Cy-NiSe and Cy-TfSe were designed and synthesized as sensitive near-infrared (NIR) fluorescent probes for detecting thiols on the basis of Se-N bond cleavage both in cells and in tissues. Since a donor-excited photoinduced electron transfer (d-PET) process occurs between the modulator and the fluorophore, Cy-NiSe and Cy-TfSe have weak fluorescence. On titration with glutathione, the free dye exhibits significant fluorescence enhancement. The two probes are sensitive and selective for thiols over other relevant biological species. They can function rapidly at pH 7.4, and their emission lies in the NIR region. Confocal imaging confirms that Cy-NiSe and Cy-TfSe can be used for detecting thiols in living cells and tissues.
学科主题Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]PHOTOINDUCED ELECTRON-TRANSFER ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; QUINONE MODIFIED ELECTRODE ; GLUTATHIONE-PEROXIDASE ; CAPILLARY-ELECTROPHORESIS ; OXIDATIVE STRESS ; HUMAN PLASMA ; ELECTROCHEMICAL DETECTION ; TOTAL HOMOCYSTEINE ; MASS-SPECTROMETRY
收录类别SCI ; IC
资助信息National Natural Science Foundation of China[20975089, 81102415]; Chinese Academy of Sciences[KZCX2-EW-206]; 100 Talents Program of the Chinese Academy of Sciences
语种英语
WOS记录号WOS:000308310100025
公开日期2013-03-08
源URL[http://ir.yic.ac.cn/handle/133337/6045]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proce, Yantai 264003, Shandong, Peoples R China
推荐引用方式
GB/T 7714
Wang, Rui,Chen, Lingxin,Liu, Ping,et al. Sensitive Near-Infrared Fluorescent Probes for Thiols Based on Se-N Bond Cleavage: Imaging in Living Cells and Tissues[J]. CHEMISTRY-A EUROPEAN JOURNAL,2012,18(36):11343-11349.
APA Wang, Rui,Chen, Lingxin,Liu, Ping,Zhang, Qin,&Wang, Yunqing.(2012).Sensitive Near-Infrared Fluorescent Probes for Thiols Based on Se-N Bond Cleavage: Imaging in Living Cells and Tissues.CHEMISTRY-A EUROPEAN JOURNAL,18(36),11343-11349.
MLA Wang, Rui,et al."Sensitive Near-Infrared Fluorescent Probes for Thiols Based on Se-N Bond Cleavage: Imaging in Living Cells and Tissues".CHEMISTRY-A EUROPEAN JOURNAL 18.36(2012):11343-11349.

入库方式: OAI收割

来源:烟台海岸带研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。