Ultrasensitive colorimetric detection of heparin based on self-assembly of gold nanoparticles on graphene oxide
文献类型：期刊论文
| 作者 | Fu, Xiuli1,2; Chen, Lingxin1 ; Li, Jinhua1
|
| 刊名 | ANALYST
 |
| 出版日期 | 2012 |
| 卷号 | 137期号:16页码:3653-3658 |
| ISSN号 | 0003-2654 |
| 关键词 | SENSING STRATEGY QUANTIFICATION CHROMATOGRAPHY SENSITIVITY SURFACE SENSOR PROBE ASSAY HG2+ |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China.,lxchen@yic.ac.cn |
| 产权排序 | [Fu, Xiuli; Chen, Lingxin; Li, Jinhua] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Fu, Xiuli] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 中文摘要 | A novel colorimetric method was developed for ultrasensitive detection of heparin based on self-assembly of gold nanoparticles (AuNPs) onto the surface of graphene oxide (GO). Polycationic protamine was used as a medium for inducing the self-assembly of citrate-capped AuNPs on GO through electrostatic interaction, resulting in a shift in the surface plasmon resonance (SPR) absorption of AuNPs and exhibiting a blue color. Addition of polyanionic heparin disturbed the self-assemble of AuNPs due to its strong affinity to protamine. With the increase of heparin concentration, the amounts of self-assembly AuNPs decreased and the color changed from blue to red in solution. Therefore, a "blue-to-red" colorimetric sensing strategy based on self-assembly of AuNPs could be established for heparin detection. Compared with the commonly reported aggregation-based methods ("red-to-blue"), the color change from blue to red was more eye-sensitive, especially in low concentration of target. Moreover, stronger interaction between protamine and heparin led to distinguish heparin from its analogues as well as various potentially coexistent physiological species. The strategy was simply achieved by the self-assembly nature of AuNPs and the application of two types of polyionic media, showing it to be label-free, simple, rapid and visual. This method could selectively detect heparin with a detection limit of 3.0 ng mL(-1) in standard aqueous solution and good linearity was obtained over the range 0.06-0.36 mu g mL(-1) (R = 0.9936). It was successfully applied to determination of heparin in fetal bovine serum samples as low as 1.7 ng mL(-1) with a linear range of 0-0.8 mu g mL(-1). |
| 英文摘要 | A novel colorimetric method was developed for ultrasensitive detection of heparin based on self-assembly of gold nanoparticles (AuNPs) onto the surface of graphene oxide (GO). Polycationic protamine was used as a medium for inducing the self-assembly of citrate-capped AuNPs on GO through electrostatic interaction, resulting in a shift in the surface plasmon resonance (SPR) absorption of AuNPs and exhibiting a blue color. Addition of polyanionic heparin disturbed the self-assemble of AuNPs due to its strong affinity to protamine. With the increase of heparin concentration, the amounts of self-assembly AuNPs decreased and the color changed from blue to red in solution. Therefore, a "blue-to-red" colorimetric sensing strategy based on self-assembly of AuNPs could be established for heparin detection. Compared with the commonly reported aggregation-based methods ("red-to-blue"), the color change from blue to red was more eye-sensitive, especially in low concentration of target. Moreover, stronger interaction between protamine and heparin led to distinguish heparin from its analogues as well as various potentially coexistent physiological species. The strategy was simply achieved by the self-assembly nature of AuNPs and the application of two types of polyionic media, showing it to be label-free, simple, rapid and visual. This method could selectively detect heparin with a detection limit of 3.0 ng mL(-1) in standard aqueous solution and good linearity was obtained over the range 0.06-0.36 mu g mL(-1) (R = 0.9936). It was successfully applied to determination of heparin in fetal bovine serum samples as low as 1.7 ng mL(-1) with a linear range of 0-0.8 mu g mL(-1). |
| 学科主题 | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | SENSING STRATEGY ; QUANTIFICATION ; CHROMATOGRAPHY ; SENSITIVITY ; SURFACE ; SENSOR ; PROBE ; ASSAY ; HG2+ |
| 资助信息 | National Natural Science Foundation of China[20975089, 21105117]; Chinese Academy of Sciences[KZCX2-EW-206]; 100 Talents Program of the Chinese Academy of Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000306483000009 |
| 公开日期 | 2013-03-08 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/6077]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Xiuli,Chen, Lingxin,Li, Jinhua. Ultrasensitive colorimetric detection of heparin based on self-assembly of gold nanoparticles on graphene oxide[J]. ANALYST,2012,137(16):3653-3658. |
| APA | Fu, Xiuli,Chen, Lingxin,&Li, Jinhua.(2012).Ultrasensitive colorimetric detection of heparin based on self-assembly of gold nanoparticles on graphene oxide.ANALYST,137(16),3653-3658. |
| MLA | Fu, Xiuli,et al."Ultrasensitive colorimetric detection of heparin based on self-assembly of gold nanoparticles on graphene oxide".ANALYST 137.16(2012):3653-3658. |
入库方式： OAI收割
来源：烟台海岸带研究所
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