Tris(hydroxymethyl)aminomethane-functionalized agarose particles: Parameters affecting the binding of bovine serum albumin
文献类型：期刊论文
| 作者 | Zhang, Bin1,2,3; Wang, Ye4; Gao, Min1; Gu, Ming1; Wang, Changhai2,4 |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2012-06-01 |
| 卷号 | 35期号:12页码:1406-1410 |
| 关键词 | Adsorption equilibrium Bovine serum albumin Protein adsorption Sepharose HP Tris(hydroxymethyl)aminomethane |
| ISSN号 | 1615-9306 |
| 产权排序 | [Zhang, Bin; Gao, Min; Gu, Ming] Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China; [Zhang, Bin; Wang, Changhai] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; [Zhang, Bin] Chinese Acad Sci, Grad Univ, Beijing 100190, Peoples R China; [Wang, Ye; Wang, Changhai] Nanjing Agr Univ, Nanjing, Jiangsu, Peoples R China |
| 通讯作者 | Gu, M (reprint author), Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China.,rainbow_gm@yahoo.com |
| 中文摘要 | A new protein adsorbent is introduced based on the coupling of the common buffer ion, tris(hydroxymethyl)aminomethane, to the agarose gel Sepharose HP from GE Healthcare Bio-Sciences AB, Uppsala, Sweden. The article describes the synthesis of the new adsorbent and the use of BSA as a model in a binding study. By optimization of the coupling procedure, a maximum ligand density of 63.5 mu mol/mL gel could be obtained. Adsorption equilibria were investigated in the pH range 5.0-8.0 and at salt concentrations of 0-0.4 mol/L. Binding of BSA under different conditions indicated that both electrostatic interaction and hydrogen bonding were involved in the adsorption process where the former played a major role. |
| 英文摘要 | A new protein adsorbent is introduced based on the coupling of the common buffer ion, tris(hydroxymethyl)aminomethane, to the agarose gel Sepharose HP from GE Healthcare Bio-Sciences AB, Uppsala, Sweden. The article describes the synthesis of the new adsorbent and the use of BSA as a model in a binding study. By optimization of the coupling procedure, a maximum ligand density of 63.5 mu mol/mL gel could be obtained. Adsorption equilibria were investigated in the pH range 5.0-8.0 and at salt concentrations of 0-0.4 mol/L. Binding of BSA under different conditions indicated that both electrostatic interaction and hydrogen bonding were involved in the adsorption process where the former played a major role. |
| 学科主题 | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | CHARGE INDUCTION CHROMATOGRAPHY ; CHELATING RESINS ; LIGAND |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China[20606032, 20636010, 50773083]; Shandong Provincial Natural Science Foundation[ZR2009BM0190A]; State Key Laboratory of Biochemical Engineering, Institute of Process Engineering[2010KF-14, 2010KF-13] |
| 语种 | 英语 |
| WOS记录号 | WOS:000306010900002 |
| 公开日期 | 2013-03-08 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/6212]  |
| 专题 | 烟台海岸带研究所_中科院烟台海岸带研究所知识产出 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100190, Peoples R China 4.Nanjing Agr Univ, Nanjing, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Bin,Wang, Ye,Gao, Min,et al. Tris(hydroxymethyl)aminomethane-functionalized agarose particles: Parameters affecting the binding of bovine serum albumin[J]. JOURNAL OF SEPARATION SCIENCE,2012,35(12):1406-1410. |
| APA | Zhang, Bin,Wang, Ye,Gao, Min,Gu, Ming,&Wang, Changhai.(2012).Tris(hydroxymethyl)aminomethane-functionalized agarose particles: Parameters affecting the binding of bovine serum albumin.JOURNAL OF SEPARATION SCIENCE,35(12),1406-1410. |
| MLA | Zhang, Bin,et al."Tris(hydroxymethyl)aminomethane-functionalized agarose particles: Parameters affecting the binding of bovine serum albumin".JOURNAL OF SEPARATION SCIENCE 35.12(2012):1406-1410. |
入库方式： OAI收割
来源：烟台海岸带研究所
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