中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Sequence of genome segments 1, 2, and 3 of the grass carp reovirus (Genus Aquareovirus, family Reoviridae)

文献类型:期刊论文

作者Fang, Q; Attoui, H; Biagini, JFP; Zhu, ZY; de Micco, P; de Lamballerie, X
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
出版日期2000-08-11
卷号274期号:3页码:762-766
关键词aquareovirus GCRV GCHV grass carp double-stranded RNA polysegmented fish reoviruses
ISSN号0006-291X
通讯作者de Lamballerie, X, Univ Mediterranee, Fac Med Marseille, Lab Virol Mol Trop & Transfus, 27 Blvd Jean Moulin, F-13005 Marseille 5, France
中文摘要The genome segments 1, 2, and 3 of the grass carp reovirus (GCRV), a tentative species assigned to genus Aquareouirus, family Reouiridae, were sequenced. The respective segments 1, 2, and 3 were 3949, 3877, and 3702 nucleotides long. Conserved moths 5' (GUUAUUU) and 3' (UUCAUC) were found at the ends of each segment. Each segment contains a single ORF and the negative strand does not permit identification of consistent ORFs. Sequence analysis revealed that VP2 is the viral polymerase, while VPI might represent the viral guanyly/methyl transferase (involved in the capping process of RNA transcripts) and VP3 the NTPase/helicase (involved in the transcription and capping of viral RNAs), The highest amino acid identities (26-41%) were found with orthoreovirus proteins. Further genomic characterization should provide insight about the genetic relationships between GCRV, aquareoviruses, and orthoreoviruses, It should also permit to precise the taxonomic status of these different viruses. (C) 2000 Academic Press.
英文摘要The genome segments 1, 2, and 3 of the grass carp reovirus (GCRV), a tentative species assigned to genus Aquareouirus, family Reouiridae, were sequenced. The respective segments 1, 2, and 3 were 3949, 3877, and 3702 nucleotides long. Conserved moths 5' (GUUAUUU) and 3' (UUCAUC) were found at the ends of each segment. Each segment contains a single ORF and the negative strand does not permit identification of consistent ORFs. Sequence analysis revealed that VP2 is the viral polymerase, while VPI might represent the viral guanyly/methyl transferase (involved in the capping process of RNA transcripts) and VP3 the NTPase/helicase (involved in the transcription and capping of viral RNAs), The highest amino acid identities (26-41%) were found with orthoreovirus proteins. Further genomic characterization should provide insight about the genetic relationships between GCRV, aquareoviruses, and orthoreoviruses, It should also permit to precise the taxonomic status of these different viruses. (C) 2000 Academic Press.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
学科主题Biochemistry & Molecular Biology; Biophysics
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]VIRUS ; RNAS
收录类别SCI
语种英语
WOS记录号WOS:000088817800033
公开日期2010-10-13
源URL[http://ir.ihb.ac.cn/handle/152342/10104]  
专题水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文
作者单位1.Univ Mediterranee, Fac Med Marseille, Lab Virol Mol Trop & Transfus, F-13005 Marseille 5, France
2.EFS Alpes Mediterranee, Unite Virus Emergents, Mol Virol Lab, F-13005 Marseille, France
3.CAS, Wuhan Inst Virol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430071, Peoples R China
4.CAS, Wuhan Inst Virol, Inst Hydrobiol, Wuhan 430071, Peoples R China
推荐引用方式
GB/T 7714
Fang, Q,Attoui, H,Biagini, JFP,et al. Sequence of genome segments 1, 2, and 3 of the grass carp reovirus (Genus Aquareovirus, family Reoviridae)[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2000,274(3):762-766.
APA Fang, Q,Attoui, H,Biagini, JFP,Zhu, ZY,de Micco, P,&de Lamballerie, X.(2000).Sequence of genome segments 1, 2, and 3 of the grass carp reovirus (Genus Aquareovirus, family Reoviridae).BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,274(3),762-766.
MLA Fang, Q,et al."Sequence of genome segments 1, 2, and 3 of the grass carp reovirus (Genus Aquareovirus, family Reoviridae)".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 274.3(2000):762-766.

入库方式: OAI收割

来源:水生生物研究所

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