Sequence of genome segments 1, 2, and 3 of the grass carp reovirus (Genus Aquareovirus, family Reoviridae)
文献类型:期刊论文
作者 | Fang, Q; Attoui, H; Biagini, JFP; Zhu, ZY; de Micco, P; de Lamballerie, X |
刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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出版日期 | 2000-08-11 |
卷号 | 274期号:3页码:762-766 |
关键词 | aquareovirus GCRV GCHV grass carp double-stranded RNA polysegmented fish reoviruses |
ISSN号 | 0006-291X |
通讯作者 | de Lamballerie, X, Univ Mediterranee, Fac Med Marseille, Lab Virol Mol Trop & Transfus, 27 Blvd Jean Moulin, F-13005 Marseille 5, France |
中文摘要 | The genome segments 1, 2, and 3 of the grass carp reovirus (GCRV), a tentative species assigned to genus Aquareouirus, family Reouiridae, were sequenced. The respective segments 1, 2, and 3 were 3949, 3877, and 3702 nucleotides long. Conserved moths 5' (GUUAUUU) and 3' (UUCAUC) were found at the ends of each segment. Each segment contains a single ORF and the negative strand does not permit identification of consistent ORFs. Sequence analysis revealed that VP2 is the viral polymerase, while VPI might represent the viral guanyly/methyl transferase (involved in the capping process of RNA transcripts) and VP3 the NTPase/helicase (involved in the transcription and capping of viral RNAs), The highest amino acid identities (26-41%) were found with orthoreovirus proteins. Further genomic characterization should provide insight about the genetic relationships between GCRV, aquareoviruses, and orthoreoviruses, It should also permit to precise the taxonomic status of these different viruses. (C) 2000 Academic Press. |
英文摘要 | The genome segments 1, 2, and 3 of the grass carp reovirus (GCRV), a tentative species assigned to genus Aquareouirus, family Reouiridae, were sequenced. The respective segments 1, 2, and 3 were 3949, 3877, and 3702 nucleotides long. Conserved moths 5' (GUUAUUU) and 3' (UUCAUC) were found at the ends of each segment. Each segment contains a single ORF and the negative strand does not permit identification of consistent ORFs. Sequence analysis revealed that VP2 is the viral polymerase, while VPI might represent the viral guanyly/methyl transferase (involved in the capping process of RNA transcripts) and VP3 the NTPase/helicase (involved in the transcription and capping of viral RNAs), The highest amino acid identities (26-41%) were found with orthoreovirus proteins. Further genomic characterization should provide insight about the genetic relationships between GCRV, aquareoviruses, and orthoreoviruses, It should also permit to precise the taxonomic status of these different viruses. (C) 2000 Academic Press. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
学科主题 | Biochemistry & Molecular Biology; Biophysics |
类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
关键词[WOS] | VIRUS ; RNAS |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000088817800033 |
公开日期 | 2010-10-13 |
源URL | [http://ir.ihb.ac.cn/handle/152342/10104] ![]() |
专题 | 水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文 |
作者单位 | 1.Univ Mediterranee, Fac Med Marseille, Lab Virol Mol Trop & Transfus, F-13005 Marseille 5, France 2.EFS Alpes Mediterranee, Unite Virus Emergents, Mol Virol Lab, F-13005 Marseille, France 3.CAS, Wuhan Inst Virol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430071, Peoples R China 4.CAS, Wuhan Inst Virol, Inst Hydrobiol, Wuhan 430071, Peoples R China |
推荐引用方式 GB/T 7714 | Fang, Q,Attoui, H,Biagini, JFP,et al. Sequence of genome segments 1, 2, and 3 of the grass carp reovirus (Genus Aquareovirus, family Reoviridae)[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2000,274(3):762-766. |
APA | Fang, Q,Attoui, H,Biagini, JFP,Zhu, ZY,de Micco, P,&de Lamballerie, X.(2000).Sequence of genome segments 1, 2, and 3 of the grass carp reovirus (Genus Aquareovirus, family Reoviridae).BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,274(3),762-766. |
MLA | Fang, Q,et al."Sequence of genome segments 1, 2, and 3 of the grass carp reovirus (Genus Aquareovirus, family Reoviridae)".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 274.3(2000):762-766. |
入库方式: OAI收割
来源:水生生物研究所
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