Heterotrophic cultivation of Chlorella kessleri for fatty acids production by carbon and nitrogen supplements
文献类型：期刊论文
| 作者 | Wang, Yan1 ; Chen, Tao2; Qin, Song1
|
| 刊名 | BIOMASS & BIOENERGY
 |
| 出版日期 | 2012-12-01 |
| 卷号 | 47页码:402-409 |
| 关键词 | Chlorella kessleri Fatty acid Nitrogen-starvation Heterotrophic |
| ISSN号 | 0961-9534 |
| 产权排序 | [Wang, Yan; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Chen, Tao] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China |
| 通讯作者 | Wang, Y (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R China.,ywang@yic.ac.cn |
| 中文摘要 | Green microalga Chlorella kessleri was grown in the dark with glucose (100 mmol l(-1)) and nitrate (10 mmol l(-1)) as initial carbon and nitrogen sources, respectively. During the cultivation, extra exogenous glucose (100 mmol l(-1)) and nitrate (10 mmol l(-1)) were singly or simultaneously supplied at interval through different strategies. Results showed that under similar concentration and frequency of glucose recharge, suitable reduction in the frequency of nitrate addition can make cells accumulate more fatty acids, while the dry biomass was still maintained at a high level. As a result, the highest biomass concentration (17.60 g l(-1)), total fatty acids content (47.67% of dry weight), and total fatty acids yield (8.39 g l(-1)) could be simultaneously obtained in the same culture, designated as intermittent C-N-feeding culture. On the other hand, since that recharge of other substrates such as fresh medium is not needed, the present intermittent C-N-feeding process is relatively easy-control and low-cost. (C) 2012 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Green microalga Chlorella kessleri was grown in the dark with glucose (100 mmol l(-1)) and nitrate (10 mmol l(-1)) as initial carbon and nitrogen sources, respectively. During the cultivation, extra exogenous glucose (100 mmol l(-1)) and nitrate (10 mmol l(-1)) were singly or simultaneously supplied at interval through different strategies. Results showed that under similar concentration and frequency of glucose recharge, suitable reduction in the frequency of nitrate addition can make cells accumulate more fatty acids, while the dry biomass was still maintained at a high level. As a result, the highest biomass concentration (17.60 g l(-1)), total fatty acids content (47.67% of dry weight), and total fatty acids yield (8.39 g l(-1)) could be simultaneously obtained in the same culture, designated as intermittent C-N-feeding culture. On the other hand, since that recharge of other substrates such as fresh medium is not needed, the present intermittent C-N-feeding process is relatively easy-control and low-cost. (C) 2012 Elsevier Ltd. All rights reserved. |
| 学科主题 | Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | LIPID-ACCUMULATION ; WASTE-WATER ; MICROALGAE ; BIODIESEL ; GROWTH ; BATCH ; VULGARIS ; OIL ; MICROORGANISMS ; STARVATION |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [50904051]; Science and Technology Planning Project of Yantai, China [2010247]; Shandong Oriental Ocean Sci-Tech Co., Ltd [200803] |
| 原文出处 | http://dx.doi.org/10.1016/j.biombioe.2012.09.018 |
| 语种 | 英语 |
| WOS记录号 | WOS:000314075900043 |
| 公开日期 | 2013-03-08 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/6115]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China 2.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yan,Chen, Tao,Qin, Song. Heterotrophic cultivation of Chlorella kessleri for fatty acids production by carbon and nitrogen supplements[J]. BIOMASS & BIOENERGY,2012,47:402-409. |
| APA | Wang, Yan,Chen, Tao,&Qin, Song.(2012).Heterotrophic cultivation of Chlorella kessleri for fatty acids production by carbon and nitrogen supplements.BIOMASS & BIOENERGY,47,402-409. |
| MLA | Wang, Yan,et al."Heterotrophic cultivation of Chlorella kessleri for fatty acids production by carbon and nitrogen supplements".BIOMASS & BIOENERGY 47(2012):402-409. |
入库方式： OAI收割
来源：烟台海岸带研究所
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