Time course of foreign gene integration and expression in transgenic fish embryos
文献类型：期刊论文
| 作者 | Zhao, HB; Chen, SP; Sun, YH; Wang, YP; Zhu, ZY |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2000-04-01 |
| 卷号 | 45期号:8页码:734-739 |
| ISSN号 | 1001-6538 |
| 关键词 | gene integration and expression time course nuclear transplantation fish GFP |
| 通讯作者 | Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Using a nuclear transplantation approach, the integration and expression of the green fluorescent protein (GFP) gene in the embryogenesis of transgenic leach (Misgurnus anguillicaudatus Cantor) have been studied. The GFP gene expression is first observed at the gastrula stage, which is consistent with the initiation of cell differentiation of fish embryos. The time course of the foreign gene expression is correlated with the regulatory sequences. The expression efficiency also depends on the gene configuration: the expression of pre-integrating circular plasmid at early embryos is higher than that of the linear plasmid. The integration of the GFP gene is first detected at the blastula stage and lasts for quite a long period. When two types of different plasmids are co-injected into fertilized eggs, the behavior of their integration and expression is not identical. |
| 英文摘要 | Using a nuclear transplantation approach, the integration and expression of the green fluorescent protein (GFP) gene in the embryogenesis of transgenic leach (Misgurnus anguillicaudatus Cantor) have been studied. The GFP gene expression is first observed at the gastrula stage, which is consistent with the initiation of cell differentiation of fish embryos. The time course of the foreign gene expression is correlated with the regulatory sequences. The expression efficiency also depends on the gene configuration: the expression of pre-integrating circular plasmid at early embryos is higher than that of the linear plasmid. The integration of the GFP gene is first detected at the blastula stage and lasts for quite a long period. When two types of different plasmids are co-injected into fertilized eggs, the behavior of their integration and expression is not identical. |
| 学科主题 | Multidisciplinary Sciences |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | GREEN FLUORESCENT PROTEIN ; GROWTH ; MICE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000087521700011 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/10122]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, HB,Chen, SP,Sun, YH,et al. Time course of foreign gene integration and expression in transgenic fish embryos[J]. CHINESE SCIENCE BULLETIN,2000,45(8):734-739. |
| APA | Zhao, HB,Chen, SP,Sun, YH,Wang, YP,&Zhu, ZY.(2000).Time course of foreign gene integration and expression in transgenic fish embryos.CHINESE SCIENCE BULLETIN,45(8),734-739. |
| MLA | Zhao, HB,et al."Time course of foreign gene integration and expression in transgenic fish embryos".CHINESE SCIENCE BULLETIN 45.8(2000):734-739. |
入库方式： OAI收割
来源：水生生物研究所
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