N-Isopropylacrylamide-Modified Polyethylenimines as Effective Gene Carriers
文献类型：期刊论文
| 作者 | Tian HY ; Li FF ; Chen J ; Huang YB ; Chen XS |
| 刊名 | macromolecular bioscience
 |
| 出版日期 | 2012 |
| 卷号 | 12期号:12页码:1680-1688 |
| 关键词 | IN-VITRO MEDIATED TRANSFECTION DNA DELIVERY PLASMID DNA THERAPY CELLS PEI VECTOR DERIVATIVES COPOLYMERS |
| ISSN号 | 1616-5187 |
| 通讯作者 | chen xs |
| 中文摘要 | 25?kda branched polyethylenimines are modified by n-isopropylacrylamide via michael addition. an agarose gel retardation assay shows that all derivatives display good binding affinity toward plasmid dna. the modified pei-25k shows lower cytotoxicity in mtt assay and better transfection efficiency than unmodified pei-25k in hela cells. the endocytosis efficiency of the optimized complexes is determined to be 99.9% by flow cytometry. more interestingly, although the derivatives are not designed to conjugate with targeting ligands or nuclear localization signals, confocal laser scanning microscopy (clsm) demonstrates that the optimized derivative results in increased endocytosis and strongly enhanced nuclear uptake compared with pei-25k. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000312242600010 |
| 公开日期 | 2013-03-17 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/47806]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Tian HY,Li FF,Chen J,et al. N-Isopropylacrylamide-Modified Polyethylenimines as Effective Gene Carriers[J]. macromolecular bioscience,2012,12(12):1680-1688. |
| APA | Tian HY,Li FF,Chen J,Huang YB,&Chen XS.(2012).N-Isopropylacrylamide-Modified Polyethylenimines as Effective Gene Carriers.macromolecular bioscience,12(12),1680-1688. |
| MLA | Tian HY,et al."N-Isopropylacrylamide-Modified Polyethylenimines as Effective Gene Carriers".macromolecular bioscience 12.12(2012):1680-1688. |
入库方式： OAI收割
来源：长春应用化学研究所
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