Improved Method for the Extraction and Determination of Bromophenols in Seafoods by High-Performance Liquid Chromatography with Fluorescence Detection
文献类型：期刊论文
| 作者 | Zhang, Shijuan1,3; Li, Yanxin2; You, Jinmao1,2; Wang, Hua2; Zheng, Yan4; Suo, Yourui1 |
| 刊名 | journal of agricultural and food chemistry
 |
| 出版日期 | 2012-11-07 |
| 卷号 | 60期号:44页码:10985-10990 |
| 关键词 | bromophenols HPLC fluorescence seafood |
| ISSN号 | 0021-8561 |
| 中文摘要 | a sensitive precolumn derivatization method using 10-methylacridone-2-sulfonyl chloride (masc) as derivatizing reagent followed by high-performance liquid chromatography (hplc) with fluorescence detection has been developed for the determination of naturally occurring bromophenols in seafoods. instead of using a traditional complex steam distillation solvent extraction method, the extraction method was modified by using sulfuric acid hydrolysis followed by hexane extraction and subsequent alkaline back extraction to obtain good recoveries and fewer interferences. batch analysis of bromophenols thus became more performable because the sample amount and analysis time were greatly reduced. the recoveries of the five bromophenols were >80% with a noticeable improvement for 4-bp, the recoveries of which were usually <40% when analyzed by traditional methods. the hplc sensitivity for the determination of bromophenols was greatly enhanced through derivatization. under optimal conditions, the quantification limits obtained by using 4.0 g of sample were in the range of 0.60-1.0 ng/g. this is the first time that derivatization was applied to enhance the hplc sensitivity of bromophenols. the proposed method was successfully applied to the determination of the target compounds in seafoods with a much higher sensitivity than traditional hplc method. |
| 英文摘要 | a sensitive precolumn derivatization method using 10-methylacridone-2-sulfonyl chloride (masc) as derivatizing reagent followed by high-performance liquid chromatography (hplc) with fluorescence detection has been developed for the determination of naturally occurring bromophenols in seafoods. instead of using a traditional complex steam distillation solvent extraction method, the extraction method was modified by using sulfuric acid hydrolysis followed by hexane extraction and subsequent alkaline back extraction to obtain good recoveries and fewer interferences. batch analysis of bromophenols thus became more performable because the sample amount and analysis time were greatly reduced. the recoveries of the five bromophenols were >80% with a noticeable improvement for 4-bp, the recoveries of which were usually <40% when analyzed by traditional methods. the hplc sensitivity for the determination of bromophenols was greatly enhanced through derivatization. under optimal conditions, the quantification limits obtained by using 4.0 g of sample were in the range of 0.60-1.0 ng/g. this is the first time that derivatization was applied to enhance the hplc sensitivity of bromophenols. the proposed method was successfully applied to the determination of the target compounds in seafoods with a much higher sensitivity than traditional hplc method. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | agriculture, multidisciplinary ; chemistry, applied ; food science & technology |
| 研究领域[WOS] | agriculture ; chemistry ; food science & technology |
| 关键词[WOS] | mass spectrometry ; enhancement ; prawns |
| 收录类别 | SCI ; IC |
| 语种 | 英语 |
| WOS记录号 | WOS:000311192200014 |
| 公开日期 | 2013-03-15 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3586]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Qufu, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Yantai Vocat Coll, Art Dept Design, Yantai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Shijuan,Li, Yanxin,You, Jinmao,et al. Improved Method for the Extraction and Determination of Bromophenols in Seafoods by High-Performance Liquid Chromatography with Fluorescence Detection[J]. journal of agricultural and food chemistry,2012,60(44):10985-10990. |
| APA | Zhang, Shijuan,Li, Yanxin,You, Jinmao,Wang, Hua,Zheng, Yan,&Suo, Yourui.(2012).Improved Method for the Extraction and Determination of Bromophenols in Seafoods by High-Performance Liquid Chromatography with Fluorescence Detection.journal of agricultural and food chemistry,60(44),10985-10990. |
| MLA | Zhang, Shijuan,et al."Improved Method for the Extraction and Determination of Bromophenols in Seafoods by High-Performance Liquid Chromatography with Fluorescence Detection".journal of agricultural and food chemistry 60.44(2012):10985-10990. |
入库方式： OAI收割
来源：西北高原生物研究所
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