Simultaneous Determination of Amino Acids in Hippophae rhamnoides L. with Pre-column Derivatization by HPLC
文献类型：期刊论文
| 作者 | Cairang, Limao1,2,3; Rui-Suo, Y.1 |
| 刊名 | asian journal of chemistry
 |
| 出版日期 | 2012-10-01 |
| 卷号 | 24期号:10页码:4380-4384 |
| 关键词 | Amino acids Hippophae rhamnoides L. HPLC Pre-column derivatization |
| ISSN号 | 0970-7077 |
| 中文摘要 | a novel, sensitive and selective hplc-flc method coupled with pre-column derivatization was developed for amino acid determination in hippophae rhamnoides l. fruit from qinghai-tibetan plateau. the method is based on the derivatization reaction of amino group with 2[2-(dibenzocarbazole)-ethoxylethyl chloroformate (dbcec) and the derivatives could be measured at 390 nm (excitation wavelength: 300 nm). detection limits (calculated as the signal-to-noise ratio 3:1) were 12.85-51.68 mol for the labeled amino acids. linearity was observed with coefficients > 0.9991 and the recoveries ranged from 92.7-105.4 %. results in this research showed that the amount of essential amino acids accounted for 70.42 % of free amino acids and 22.68 % of the total amino acids, respectively, which benefits people's health through drinking the juice or eating the related products. proline was dominating among free amino acids in samples, which plays a vital role for hippophae rhamnoides l. in resisting to drought, tolerating soil salinity and lowing temperature in qinghai-tibetan plateau. |
| 英文摘要 | a novel, sensitive and selective hplc-flc method coupled with pre-column derivatization was developed for amino acid determination in hippophae rhamnoides l. fruit from qinghai-tibetan plateau. the method is based on the derivatization reaction of amino group with 2[2-(dibenzocarbazole)-ethoxylethyl chloroformate (dbcec) and the derivatives could be measured at 390 nm (excitation wavelength: 300 nm). detection limits (calculated as the signal-to-noise ratio 3:1) were 12.85-51.68 mol for the labeled amino acids. linearity was observed with coefficients > 0.9991 and the recoveries ranged from 92.7-105.4 %. results in this research showed that the amount of essential amino acids accounted for 70.42 % of free amino acids and 22.68 % of the total amino acids, respectively, which benefits people's health through drinking the juice or eating the related products. proline was dominating among free amino acids in samples, which plays a vital role for hippophae rhamnoides l. in resisting to drought, tolerating soil salinity and lowing temperature in qinghai-tibetan plateau. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | performance liquid-chromatography ; ionization-mass-spectrometry ; fluorescence detection ; identification ; reagent |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000310771000031 |
| 公开日期 | 2013-03-15 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3598]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Qinghai Normal Univ, Coll Nationality Normal, Dept Chem Sci, Xining, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cairang, Limao,Rui-Suo, Y.. Simultaneous Determination of Amino Acids in Hippophae rhamnoides L. with Pre-column Derivatization by HPLC[J]. asian journal of chemistry,2012,24(10):4380-4384. |
| APA | Cairang, Limao,&Rui-Suo, Y..(2012).Simultaneous Determination of Amino Acids in Hippophae rhamnoides L. with Pre-column Derivatization by HPLC.asian journal of chemistry,24(10),4380-4384. |
| MLA | Cairang, Limao,et al."Simultaneous Determination of Amino Acids in Hippophae rhamnoides L. with Pre-column Derivatization by HPLC".asian journal of chemistry 24.10(2012):4380-4384. |
入库方式： OAI收割
来源：西北高原生物研究所
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