Fluorescence Probe of 10-Phenyl-acridone-2-sulfonyl Chloride and Its Application for Determination of Free Aliphatic Amines in Environmental Samples by HPLC with Fluorescence Detection and APCI-MS
文献类型：期刊论文
| 作者 | Ning, Shujing1,2; You, Jinmao1,3; Sun, Zhiwei1; Zhang, Shijuan3; Ji, Zhongyin3 |
| 刊名 | chromatographia
 |
| 出版日期 | 2012-10-01 |
| 卷号 | 75期号:19-20页码:1107-1116 |
| 关键词 | HPLC/MS Aliphatic amines 10-Phenyl-acridone-2-sulfonyl chloride Pre-column derivatization |
| ISSN号 | 0009-5893 |
| 中文摘要 | a simple and sensitive method for the determination of free aliphatic amines using 10-phenyl-acridone-2-sulfonyl chloride (pasc) as a labeling reagent by high-performance liquid chromatography with fluorescence detection and online mass spectrometry identification (hplc-fld-ms) has been developed. derivatization conditions including reagent concentration, buffer ph, reaction time and temperature were optimized. pasc reacted with aliphatic amines at 50 a degrees c for 4 min in aqueous acetonitrile (acn) in the presence of sodiumtetraborate-naoh buffer (0.10 mol l-1, ph 9.0) to give high yields of pasc-amine derivatives. derivatives exhibited intense fluorescence with an excitation maximum at lambda(ex) 265 nm and an emission maximum at lambda(em) 418 nm. the separation of derivatives was performed by a reversed-phase hypersil bds c8 column in combination with a gradient elution. the identification of derivatives was carried out by online post-column mass spectrometry with atmospheric pressure chemical ionization (apci) source in positive-ion detection mode. excellent linear responses were observed with the correlation coefficients of larger than 0.9997, and detection limits (at a signal-to-noise of 3:1) were from 3.0 to 24.3 fmol. comparing with 10-ethyl-acridine-2-sulfonyl chloride (easc), pasc exhibited more intense fluorescence and ultraviolet absorbance. the proposed method is sensitive and reproducible for the determination of aliphatic amines from water and soil samples. |
| 英文摘要 | a simple and sensitive method for the determination of free aliphatic amines using 10-phenyl-acridone-2-sulfonyl chloride (pasc) as a labeling reagent by high-performance liquid chromatography with fluorescence detection and online mass spectrometry identification (hplc-fld-ms) has been developed. derivatization conditions including reagent concentration, buffer ph, reaction time and temperature were optimized. pasc reacted with aliphatic amines at 50 a degrees c for 4 min in aqueous acetonitrile (acn) in the presence of sodiumtetraborate-naoh buffer (0.10 mol l-1, ph 9.0) to give high yields of pasc-amine derivatives. derivatives exhibited intense fluorescence with an excitation maximum at lambda(ex) 265 nm and an emission maximum at lambda(em) 418 nm. the separation of derivatives was performed by a reversed-phase hypersil bds c8 column in combination with a gradient elution. the identification of derivatives was carried out by online post-column mass spectrometry with atmospheric pressure chemical ionization (apci) source in positive-ion detection mode. excellent linear responses were observed with the correlation coefficients of larger than 0.9997, and detection limits (at a signal-to-noise of 3:1) were from 3.0 to 24.3 fmol. comparing with 10-ethyl-acridine-2-sulfonyl chloride (easc), pasc exhibited more intense fluorescence and ultraviolet absorbance. the proposed method is sensitive and reproducible for the determination of aliphatic amines from water and soil samples. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; pressure chemical-ionization ; volatile n-nitrosamines ; biogenic-amines ; gas-chromatography ; precolumn derivatization ; enzymatic method ; polyamines ; chloroformate ; products |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000309223800003 |
| 公开日期 | 2013-03-15 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3606]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273156, Peoples R China 2.Expt High Sch Linqu, Linqu 262600, Peoples R China 3.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ning, Shujing,You, Jinmao,Sun, Zhiwei,et al. Fluorescence Probe of 10-Phenyl-acridone-2-sulfonyl Chloride and Its Application for Determination of Free Aliphatic Amines in Environmental Samples by HPLC with Fluorescence Detection and APCI-MS[J]. chromatographia,2012,75(19-20):1107-1116. |
| APA | Ning, Shujing,You, Jinmao,Sun, Zhiwei,Zhang, Shijuan,&Ji, Zhongyin.(2012).Fluorescence Probe of 10-Phenyl-acridone-2-sulfonyl Chloride and Its Application for Determination of Free Aliphatic Amines in Environmental Samples by HPLC with Fluorescence Detection and APCI-MS.chromatographia,75(19-20),1107-1116. |
| MLA | Ning, Shujing,et al."Fluorescence Probe of 10-Phenyl-acridone-2-sulfonyl Chloride and Its Application for Determination of Free Aliphatic Amines in Environmental Samples by HPLC with Fluorescence Detection and APCI-MS".chromatographia 75.19-20(2012):1107-1116. |
入库方式： OAI收割
来源：西北高原生物研究所
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