Analysis of free fatty acids in Notopterygium forbesii Boiss by a novel HPLC method with fluorescence detection
文献类型：期刊论文
| 作者 | Zhang, Shijuan1,3; You, Jinmao1,2; Zhou, Guoying1; Li, Chunli1,3; Suo, Yourui1 |
| 刊名 | talanta
 |
| 出版日期 | 2012-08-30 |
| 卷号 | 98页码:95-100 |
| 关键词 | Notopterygium forbesii Boiss Fatty acids Fluorescence HPLC |
| ISSN号 | 0039-9140 |
| 中文摘要 | a new labeling reagent for fatty acids, 1-(9h-carbazol-9-yl) propan-2-yl-methanesulfonate (cpms), has been synthesized and successfully applied to the hplc determination of fatty acids in traditional chinese herb notopterygium forbesii boiss. the reaction of cpms with fatty acids could proceed easily and quickly in the presence of k2co3 catalyst within 30 min. the derivatives exhibit excellent fluorescence property with excitation and emission wavelengths of 293 nm and 360 nm respectively. the 34 derivatives of fatty acids were separated on a bds c8 reversed-phase column with gradient elution. good linear correlations were observed for all fatty acids with correlation coefficients of > 0.996. the detection limits at a signal-to-noise ratio of 3 were in the range 0.032-0.312 mu g g(-1). free fatty acids in the roots, stem, leaves and petioles of n. forbesii boiss from different places were analyzed by the developed method. this is the first time that the fatty acids composition of n. forbesii boiss has been reported. this method also shows powerful potential for the trace analysis of fatty acids or other carboxylic acids from complex samples. (c) 2012 elsevier b.v. all rights reserved. |
| 英文摘要 | a new labeling reagent for fatty acids, 1-(9h-carbazol-9-yl) propan-2-yl-methanesulfonate (cpms), has been synthesized and successfully applied to the hplc determination of fatty acids in traditional chinese herb notopterygium forbesii boiss. the reaction of cpms with fatty acids could proceed easily and quickly in the presence of k2co3 catalyst within 30 min. the derivatives exhibit excellent fluorescence property with excitation and emission wavelengths of 293 nm and 360 nm respectively. the 34 derivatives of fatty acids were separated on a bds c8 reversed-phase column with gradient elution. good linear correlations were observed for all fatty acids with correlation coefficients of > 0.996. the detection limits at a signal-to-noise ratio of 3 were in the range 0.032-0.312 mu g g(-1). free fatty acids in the roots, stem, leaves and petioles of n. forbesii boiss from different places were analyzed by the developed method. this is the first time that the fatty acids composition of n. forbesii boiss has been reported. this method also shows powerful potential for the trace analysis of fatty acids or other carboxylic acids from complex samples. (c) 2012 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | performance liquid-chromatography ; gas-chromatography ; mass-spectrometry ; risk-factors ; fish-oil ; extraction ; omega-3-fatty-acids ; quantification ; identification ; quantitation |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000309327600014 |
| 公开日期 | 2013-03-15 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3618]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Qufu, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Shijuan,You, Jinmao,Zhou, Guoying,et al. Analysis of free fatty acids in Notopterygium forbesii Boiss by a novel HPLC method with fluorescence detection[J]. talanta,2012,98:95-100. |
| APA | Zhang, Shijuan,You, Jinmao,Zhou, Guoying,Li, Chunli,&Suo, Yourui.(2012).Analysis of free fatty acids in Notopterygium forbesii Boiss by a novel HPLC method with fluorescence detection.talanta,98,95-100. |
| MLA | Zhang, Shijuan,et al."Analysis of free fatty acids in Notopterygium forbesii Boiss by a novel HPLC method with fluorescence detection".talanta 98(2012):95-100. |
入库方式： OAI收割
来源：西北高原生物研究所
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