Determination of fatty acids from mushrooms using high performance liquid chromatography with fluorescence detection and online mass spectrometry
文献类型：期刊论文
| 作者 | Jing, Nianhua1,3; Shi, Junyou2; Li, Guoliang4; Sun, Zhiwei4; You, Jinmao1,4 |
| 刊名 | food research international
 |
| 出版日期 | 2012-08-01 |
| 卷号 | 48期号:1页码:155-163 |
| 关键词 | Fatty acids Armillaria luteo-virens HPLC-FLD-MS/MS Pre-column derivatization |
| ISSN号 | 0963-9969 |
| 中文摘要 | owing to the growing consumer interest in low-fat foods, it is necessary to provide our customers with the information about the fatty acid composition of mushrooms. in this study, a selective and sensitive method based on pre-column derivatization using 2-(12-oxobenzo[b]-acridin-5(12h)-yl)-ethyl-4-toluenesulfonate (baets) as the labeling reagent has been optimized by high-performance liquid chromatography with fluorescence detection and online mass spectrometry identification (hplc-fld-ms/ms). fatty acids (fa) were derivatized by baets and separated on a reversed-phase hypersil bds c8 column with a gradient elution. eighteen fa investigated were found to give excellent linear responses with correlation coefficients of >0.9996. limits of detection and quantification (lod and loq) were in the range of 0.46 to 1.02 ng ml(-1) and 1.43 to 3.48 ng ml(-1), respectively. this method was applied to the quantitative analysis of fa from a wild and four cultivated mushroom species. the wild mushroom named armillaria luteo-virens contained higher unsaturated fatty acids (ufa) when compared to the cultivated species (flammulina velutiper, pleurotus eryngii, copyinds comatus and agrocybe aegerita). ratio of ufa:sfa (sfa, saturated fatty acids) for a. luteo-virens was >5 whereas the values for the cultivated species were <4.09. therefore, baets derivatization allowed the development of a highly sensitive and specific method for the determination of fa in mushroom samples. (c) 2012 elsevier ltd. all rights reserved. |
| 英文摘要 | owing to the growing consumer interest in low-fat foods, it is necessary to provide our customers with the information about the fatty acid composition of mushrooms. in this study, a selective and sensitive method based on pre-column derivatization using 2-(12-oxobenzo[b]-acridin-5(12h)-yl)-ethyl-4-toluenesulfonate (baets) as the labeling reagent has been optimized by high-performance liquid chromatography with fluorescence detection and online mass spectrometry identification (hplc-fld-ms/ms). fatty acids (fa) were derivatized by baets and separated on a reversed-phase hypersil bds c8 column with a gradient elution. eighteen fa investigated were found to give excellent linear responses with correlation coefficients of >0.9996. limits of detection and quantification (lod and loq) were in the range of 0.46 to 1.02 ng ml(-1) and 1.43 to 3.48 ng ml(-1), respectively. this method was applied to the quantitative analysis of fa from a wild and four cultivated mushroom species. the wild mushroom named armillaria luteo-virens contained higher unsaturated fatty acids (ufa) when compared to the cultivated species (flammulina velutiper, pleurotus eryngii, copyinds comatus and agrocybe aegerita). ratio of ufa:sfa (sfa, saturated fatty acids) for a. luteo-virens was >5 whereas the values for the cultivated species were <4.09. therefore, baets derivatization allowed the development of a highly sensitive and specific method for the determination of fa in mushroom samples. (c) 2012 elsevier ltd. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | food science & technology |
| 研究领域[WOS] | food science & technology |
| 关键词[WOS] | wild edible mushrooms ; american-heart-association ; armillaria-luteo-virens ; cardiovascular-disease ; electrospray-ionization ; gas-chromatography ; nutritional-value ; amino-acids ; identification ; extraction |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000307430100019 |
| 公开日期 | 2013-03-15 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3632]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Qujing Normal Univ, Coll Chem Sci, Qujing, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 4.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jing, Nianhua,Shi, Junyou,Li, Guoliang,et al. Determination of fatty acids from mushrooms using high performance liquid chromatography with fluorescence detection and online mass spectrometry[J]. food research international,2012,48(1):155-163. |
| APA | Jing, Nianhua,Shi, Junyou,Li, Guoliang,Sun, Zhiwei,&You, Jinmao.(2012).Determination of fatty acids from mushrooms using high performance liquid chromatography with fluorescence detection and online mass spectrometry.food research international,48(1),155-163. |
| MLA | Jing, Nianhua,et al."Determination of fatty acids from mushrooms using high performance liquid chromatography with fluorescence detection and online mass spectrometry".food research international 48.1(2012):155-163. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。