Preparative Separation of Methylswertianin, Swerchirin and Decussatin from the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography
文献类型：期刊论文
| 作者 | Jia, Jing1,2; Chen, Tao1,2; Wang, Ping1,2; Chen, Guichen1; You, Jinmao1; Liu, Yongjun1; Li, Yulin1 |
| 刊名 | phytochemical analysis
 |
| 出版日期 | 2012-07-01 |
| 卷号 | 23期号:4页码:332-336 |
| 关键词 | high-speed counter-current chromatography xanthone methylswertianin swerchirin decussatin Swertia mussotii |
| ISSN号 | 0958-0344 |
| 中文摘要 | introduction xanthones, the primary constituents of swertia mussotii, are known to possess a variety of biological activities, including anti-depressant, anti-leukaemic, anti-tumour, anti-tubercular, choleretic, diuretic, anti-microbial, anti-fungal, anti-inflammatory, anti-viral, cardiotonic and hypoglycemic properties. however, high performance, environmentally friendly methods for isolating and purifying xanthones from s. mussotii are not currently available. objective to develop a high performance and environmentally friendly method for the preparative separation of xanthones methylswertianin, swerchirin and decussatin from s. mussotii using high-speed counter-current chromatography (hsccc). methodology a solvent system composed of n-hexane:ethyl acatate:methanol:water (5:5:10:4, v/v/v/v) was developed for the separation method. the upper phase was used as the stationary phase, and the lower phase was used as the mobile phase at a flow rate of 1.5?ml/min, a rotation speed of 800?rpm and a temperature of 25 degrees c. results using the described method, 8?mg of methylswertianin, 21?mg of swerchirin and 11?mg of decussatin with purities of over 98% could be isolated from a 150?mg crude sample. they were identified by 1h-nmr and 13?c-nmr analysis. conclusion three xanthones in swertia mussotii could be systematically isolated and purified using hsccc. copyright (c) 2011 john wiley & sons, ltd. |
| 英文摘要 | introduction xanthones, the primary constituents of swertia mussotii, are known to possess a variety of biological activities, including anti-depressant, anti-leukaemic, anti-tumour, anti-tubercular, choleretic, diuretic, anti-microbial, anti-fungal, anti-inflammatory, anti-viral, cardiotonic and hypoglycemic properties. however, high performance, environmentally friendly methods for isolating and purifying xanthones from s. mussotii are not currently available. objective to develop a high performance and environmentally friendly method for the preparative separation of xanthones methylswertianin, swerchirin and decussatin from s. mussotii using high-speed counter-current chromatography (hsccc). methodology a solvent system composed of n-hexane:ethyl acatate:methanol:water (5:5:10:4, v/v/v/v) was developed for the separation method. the upper phase was used as the stationary phase, and the lower phase was used as the mobile phase at a flow rate of 1.5?ml/min, a rotation speed of 800?rpm and a temperature of 25 degrees c. results using the described method, 8?mg of methylswertianin, 21?mg of swerchirin and 11?mg of decussatin with purities of over 98% could be isolated from a 150?mg crude sample. they were identified by 1h-nmr and 13?c-nmr analysis. conclusion three xanthones in swertia mussotii could be systematically isolated and purified using hsccc. copyright (c) 2011 john wiley & sons, ltd. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; plant sciences ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; plant sciences ; chemistry |
| 关键词[WOS] | purification ; xanthones ; isopsoralen ; psoralen ; radix |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000305571500007 |
| 公开日期 | 2013-03-15 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3652]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jia, Jing,Chen, Tao,Wang, Ping,et al. Preparative Separation of Methylswertianin, Swerchirin and Decussatin from the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography[J]. phytochemical analysis,2012,23(4):332-336. |
| APA | Jia, Jing.,Chen, Tao.,Wang, Ping.,Chen, Guichen.,You, Jinmao.,...&Li, Yulin.(2012).Preparative Separation of Methylswertianin, Swerchirin and Decussatin from the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography.phytochemical analysis,23(4),332-336. |
| MLA | Jia, Jing,et al."Preparative Separation of Methylswertianin, Swerchirin and Decussatin from the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography".phytochemical analysis 23.4(2012):332-336. |
入库方式： OAI收割
来源：西北高原生物研究所
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