A sensitive and selective HPLC-FLD method with fluorescent labeling for simultaneous detection of bile acid and free fatty acid in human serum
文献类型：期刊论文
| 作者 | Li, Guo-Liang1; Chen, Guang1,2; Liu, Yue-Qiu3; Jing, Nian-Hua2; You, Jin-Mao1,2 |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2012-05-01 |
| 卷号 | 895页码:191-195 |
| 关键词 | Bile acids Free fatty acids Human serum Fluorescent labeling HPLC-FLD |
| ISSN号 | 1570-0232 |
| 中文摘要 | a sensitive and selective method using 2(7h-dibenzo[a,g]carbazol-7-yl)ethyl 4-methylbenzenesulfonate (dbcets) as a new fluorescent labeling reagent has been proposed for simultaneously detecting ba and ffa by hplc with fluorescence detector. the developed method offered the low detection limits of 0.42-0.70 and 0.28-0.57 ng/ml for ba and ffa, respectively. compared with the reported methods, the proposed method here is capable of offering higher detection sensitivity and selectivity, with less cost and lower volume of sample preparation. this method was validated to ensure high accuracy and precision, and the reliability of its results. when applied to the serum samples of healthy volunteers and patients with hepatic carcinoma, it showed excellent applicability. (c) 2012 elsevier b.v. all rights reserved. |
| 英文摘要 | a sensitive and selective method using 2(7h-dibenzo[a,g]carbazol-7-yl)ethyl 4-methylbenzenesulfonate (dbcets) as a new fluorescent labeling reagent has been proposed for simultaneously detecting ba and ffa by hplc with fluorescence detector. the developed method offered the low detection limits of 0.42-0.70 and 0.28-0.57 ng/ml for ba and ffa, respectively. compared with the reported methods, the proposed method here is capable of offering higher detection sensitivity and selectivity, with less cost and lower volume of sample preparation. this method was validated to ensure high accuracy and precision, and the reliability of its results. when applied to the serum samples of healthy volunteers and patients with hepatic carcinoma, it showed excellent applicability. (c) 2012 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; tandem mass-spectrometry ; solid-phase extraction ; carboxylic-acids ; capillary-electrophoresis ; derivatization method ; ursodeoxycholic acid ; biological-fluids ; plasma ; reagent |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000303628700029 |
| 公开日期 | 2013-03-15 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3684]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Key Lab Lifeorgan Anal Shandong Prov, Qufu, Peoples R China 2.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Evolut & Adaptat Plateau Biota, Xining 810001, Peoples R China 3.Sichuan Agr Univ, Chengdu 611130, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Guo-Liang,Chen, Guang,Liu, Yue-Qiu,et al. A sensitive and selective HPLC-FLD method with fluorescent labeling for simultaneous detection of bile acid and free fatty acid in human serum[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2012,895:191-195. |
| APA | Li, Guo-Liang,Chen, Guang,Liu, Yue-Qiu,Jing, Nian-Hua,&You, Jin-Mao.(2012).A sensitive and selective HPLC-FLD method with fluorescent labeling for simultaneous detection of bile acid and free fatty acid in human serum.journal of chromatography b-analytical technologies in the biomedical and life sciences,895,191-195. |
| MLA | Li, Guo-Liang,et al."A sensitive and selective HPLC-FLD method with fluorescent labeling for simultaneous detection of bile acid and free fatty acid in human serum".journal of chromatography b-analytical technologies in the biomedical and life sciences 895(2012):191-195. |
入库方式： OAI收割
来源：西北高原生物研究所
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