Differential gene expression of protein kinases in oocytes between natural gynogenetic silver crucian carp and amphimictic crucian carp
文献类型：期刊论文
| 作者 | Xie, J; Zhu, Y; Zhang, F; Gui, JF |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 1999-07-01 |
| 卷号 | 44期号:14页码:1297-1301 |
| 关键词 | mRNA differential display gynogenesis protein kinase silver crucian carp |
| ISSN号 | 1001-6538 |
| 通讯作者 | Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | A modified mRNA differential display method has been applied to studying differential expression of protein kinase genes in oocytes between natural gynogenetic silver crucian carp and amphimictic crucian carp. Total RNA was reverse transcribed using downstream 3' primers T(12)MA, T(12)MG and T12MC respectively. Then the reverse transcription products were amplified using upstream 5' kinase-specific primer designed according to protein kinase conserved sequence. The PCR products had different patterns and numbers of: cDNA bands on polyacrylamide:gel. Totally 21 cDNAs fragments were recovered and cloned. Two of them were confirmed to be particularly expressed in oocytes of amphimictic crucian carp, and another was specific for gynogenetic silver crucian carp. |
| 英文摘要 | A modified mRNA differential display method has been applied to studying differential expression of protein kinase genes in oocytes between natural gynogenetic silver crucian carp and amphimictic crucian carp. Total RNA was reverse transcribed using downstream 3' primers T(12)MA, T(12)MG and T12MC respectively. Then the reverse transcription products were amplified using upstream 5' kinase-specific primer designed according to protein kinase conserved sequence. The PCR products had different patterns and numbers of: cDNA bands on polyacrylamide:gel. Totally 21 cDNAs fragments were recovered and cloned. Two of them were confirmed to be particularly expressed in oocytes of amphimictic crucian carp, and another was specific for gynogenetic silver crucian carp. |
| WOS标题词 | Science & Technology |
| 学科主题 | Multidisciplinary Sciences |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | CELL-SURFACE MOLECULE ; MESSENGER-RNA ; FERTILIZATION ; ACTIVATION ; DISPLAY ; FAMILY ; PLEXIN ; SPERM ; EGG |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000083011200010 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/10172]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Wuhan Univ, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, J,Zhu, Y,Zhang, F,et al. Differential gene expression of protein kinases in oocytes between natural gynogenetic silver crucian carp and amphimictic crucian carp[J]. CHINESE SCIENCE BULLETIN,1999,44(14):1297-1301. |
| APA | Xie, J,Zhu, Y,Zhang, F,&Gui, JF.(1999).Differential gene expression of protein kinases in oocytes between natural gynogenetic silver crucian carp and amphimictic crucian carp.CHINESE SCIENCE BULLETIN,44(14),1297-1301. |
| MLA | Xie, J,et al."Differential gene expression of protein kinases in oocytes between natural gynogenetic silver crucian carp and amphimictic crucian carp".CHINESE SCIENCE BULLETIN 44.14(1999):1297-1301. |
入库方式： OAI收割
来源：水生生物研究所
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