PREPARATIVE ISOLATION AND PURIFICATION OF LUTONARIN AND SAPONARIN FROM BARLEY SEEDLINGS BY HSCCC
文献类型：期刊论文
| 作者 | Chen, Tao1,2; Wang, Ping1,2; Du, Yuzhi1; Shen, Yuhu1; Li, Yulin1 |
| 刊名 | journal of liquid chromatography & related technologies
 |
| 出版日期 | 2012 |
| 卷号 | 35期号:18页码:2524-2532 |
| 关键词 | barley seedlings Hordeum vulgare HPLC HSCCC lutonarin saponarin |
| ISSN号 | 1082-6076 |
| 中文摘要 | in order to isolate large amounts of pure lutonarin and saponarin, a high performance method for isolation and purification of these two flavonoids from barley (hordeum vulgare) seedlings was successfully established by utilizing high-speed counter-current chromatography (hsccc). separation was performed with a two-phase solvent system: ethyl acetate/n-butanol/water (3:2:5, v/v/v). a total of 24 mg lutonarin and 14 mg saponarin was obtained in one-step separation from 100 mg sample. high performance liquid chromatography (hplc) analysis showed that the purity of each of the two compounds was over 98%. their chemical structures were confirmed by uv, h-1 nmr, and c-13 nmr data. |
| 英文摘要 | in order to isolate large amounts of pure lutonarin and saponarin, a high performance method for isolation and purification of these two flavonoids from barley (hordeum vulgare) seedlings was successfully established by utilizing high-speed counter-current chromatography (hsccc). separation was performed with a two-phase solvent system: ethyl acetate/n-butanol/water (3:2:5, v/v/v). a total of 24 mg lutonarin and 14 mg saponarin was obtained in one-step separation from 100 mg sample. high performance liquid chromatography (hplc) analysis showed that the purity of each of the two compounds was over 98%. their chemical structures were confirmed by uv, h-1 nmr, and c-13 nmr data. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | speed countercurrent chromatography ; liquid-chromatography ; hordeum-vulgare ; leaves ; flavonoids ; glycosides |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000310738000002 |
| 公开日期 | 2013-03-15 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3736]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Qinghai Prov, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Tao,Wang, Ping,Du, Yuzhi,et al. PREPARATIVE ISOLATION AND PURIFICATION OF LUTONARIN AND SAPONARIN FROM BARLEY SEEDLINGS BY HSCCC[J]. journal of liquid chromatography & related technologies,2012,35(18):2524-2532. |
| APA | Chen, Tao,Wang, Ping,Du, Yuzhi,Shen, Yuhu,&Li, Yulin.(2012).PREPARATIVE ISOLATION AND PURIFICATION OF LUTONARIN AND SAPONARIN FROM BARLEY SEEDLINGS BY HSCCC.journal of liquid chromatography & related technologies,35(18),2524-2532. |
| MLA | Chen, Tao,et al."PREPARATIVE ISOLATION AND PURIFICATION OF LUTONARIN AND SAPONARIN FROM BARLEY SEEDLINGS BY HSCCC".journal of liquid chromatography & related technologies 35.18(2012):2524-2532. |
入库方式： OAI收割
来源：西北高原生物研究所
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