DETERMINATION AND IDENTIFICATION OF FATTY ACIDS IN MICROULA SIKKIMENSIS SEED OIL USING 1,2-BENZOCARBAZOLE-9-ETHYL-P-TOLUENESULFONATE AS A NOVEL LABELING REAGENT BY HPLC WITH FLUORESCENCE DETECTION AND APCI-MS
文献类型：期刊论文
| 作者 | Song, Cuihua1; Zhang, Caiqing2; Sun, Zhiwei1; You, Jinmao1,3; Suo, Yourui3 |
| 刊名 | journal of liquid chromatography & related technologies
 |
| 出版日期 | 2012 |
| 卷号 | 35期号:14页码:2066-2080 |
| 关键词 | derivatization fatty acids fluorescence detection high performance liquid chromatography mass spectrometry Microula sikkimensis Hemsl seed oil |
| ISSN号 | 1082-6076 |
| 中文摘要 | using 1,2-benzocarbazole-9-ethyl-p-toluenesulfonate (bcets) as the labeling reagent, a sensitive method for the determination of fatty acids by high performance liquid chromatography (hplc) with fluorescence detection has been developed. the bcets could easily and quickly label fatty acids in the presence of the k2co3 catalyst at 90 degrees c for 30 min in n,n-dimethylformamide solvent. all the tested fatty acid derivatives were separated on a reversed-phase eclipse xdb-c-8 column by hplc in conjunction with gradient elution. the identification was carried out by post-column atmospheric pressure chemical ionization mass spectrometry (apci/ms) in positive ion detection mode. the fluorescence excitation and emission wavelengths of the derivatives were at lambda(ex) 279 nm and lambda(em) 380 nm, respectively. linear correlation coefficients for all fatty acid derivatives are more than 0.9994. detection limits, at a signal-to-noise ratio of 3:1, were 0.21-0.99 mu g/l for the labeled fatty acids. in this study, fatty acids from the extracted microula sikkimensis hemsl (m. sikkimensis h.) seed were sensitively determined. it was found that the seed oil mainly contained oleic acid, linoleic acid, alpha-linolenic acid, hexadecanoic acid, stearidonic acid, gamma-linolenic acid, and eicosenoic acid. m. sikkimensis h. seed oil was rich in unsaturated fatty acids and may be used as high-quality edible oil for its health benefits. |
| 英文摘要 | using 1,2-benzocarbazole-9-ethyl-p-toluenesulfonate (bcets) as the labeling reagent, a sensitive method for the determination of fatty acids by high performance liquid chromatography (hplc) with fluorescence detection has been developed. the bcets could easily and quickly label fatty acids in the presence of the k2co3 catalyst at 90 degrees c for 30 min in n,n-dimethylformamide solvent. all the tested fatty acid derivatives were separated on a reversed-phase eclipse xdb-c-8 column by hplc in conjunction with gradient elution. the identification was carried out by post-column atmospheric pressure chemical ionization mass spectrometry (apci/ms) in positive ion detection mode. the fluorescence excitation and emission wavelengths of the derivatives were at lambda(ex) 279 nm and lambda(em) 380 nm, respectively. linear correlation coefficients for all fatty acid derivatives are more than 0.9994. detection limits, at a signal-to-noise ratio of 3:1, were 0.21-0.99 mu g/l for the labeled fatty acids. in this study, fatty acids from the extracted microula sikkimensis hemsl (m. sikkimensis h.) seed were sensitively determined. it was found that the seed oil mainly contained oleic acid, linoleic acid, alpha-linolenic acid, hexadecanoic acid, stearidonic acid, gamma-linolenic acid, and eicosenoic acid. m. sikkimensis h. seed oil was rich in unsaturated fatty acids and may be used as high-quality edible oil for its health benefits. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; carboxylic-acids ; trifluoromethanesulfonate ; bryophyte ; pollen |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000308024300011 |
| 公开日期 | 2013-03-15 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/3744]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal Shandong Pr, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China 2.Shandong Prov Qianfoshan Hosp, Jinan, Peoples R China 3.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Cuihua,Zhang, Caiqing,Sun, Zhiwei,et al. DETERMINATION AND IDENTIFICATION OF FATTY ACIDS IN MICROULA SIKKIMENSIS SEED OIL USING 1,2-BENZOCARBAZOLE-9-ETHYL-P-TOLUENESULFONATE AS A NOVEL LABELING REAGENT BY HPLC WITH FLUORESCENCE DETECTION AND APCI-MS[J]. journal of liquid chromatography & related technologies,2012,35(14):2066-2080. |
| APA | Song, Cuihua,Zhang, Caiqing,Sun, Zhiwei,You, Jinmao,&Suo, Yourui.(2012).DETERMINATION AND IDENTIFICATION OF FATTY ACIDS IN MICROULA SIKKIMENSIS SEED OIL USING 1,2-BENZOCARBAZOLE-9-ETHYL-P-TOLUENESULFONATE AS A NOVEL LABELING REAGENT BY HPLC WITH FLUORESCENCE DETECTION AND APCI-MS.journal of liquid chromatography & related technologies,35(14),2066-2080. |
| MLA | Song, Cuihua,et al."DETERMINATION AND IDENTIFICATION OF FATTY ACIDS IN MICROULA SIKKIMENSIS SEED OIL USING 1,2-BENZOCARBAZOLE-9-ETHYL-P-TOLUENESULFONATE AS A NOVEL LABELING REAGENT BY HPLC WITH FLUORESCENCE DETECTION AND APCI-MS".journal of liquid chromatography & related technologies 35.14(2012):2066-2080. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。