Studies on morphogenesis and cellular interactions of Rana grylio virus in an infected fish cell line
文献类型：期刊论文
| 作者 | Zhang, QY; Li, ZQ; Gui, JF |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 1999-05-15 |
| 卷号 | 175期号:3-4页码:185-197 |
| ISSN号 | 0044-8486 |
| 关键词 | Rana grylio RGV iridovirus morphogenesis fish cell line |
| 通讯作者 | Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | A pathogenic virus (RGV), isolated from diseased pig frog Rana grylio with lethal syndrome, was investigated with regard to morphogenesis and cellular interactions in EPC cells, a cell Line from fish. Different stages of virus amplification, maturation and assembly were observed at nucleus, cytoplasm and cellular membranes. The matured virus particles, were not only distributed diffusely in nucleus, cytoplasm and cellular surface, but also aggregated as pseudocrystalline arrays in the cytoplasm. Virions were released by budding from the plasma membranes, or following cell lysis. Various types of cell damage, such as small vacuoles, spherical inclusions, and swollen and empty mitochondria, were also found. Some typical characteristics of RGV, such as the symmetrical shape of the virions, replication process involving both nuclear and cytoplasmic phases, budding release from cellular membrane and intracellular membrane, viromatrix and paracrystalline aggregation in cytoplasm, and its acute pathogenic effects, were observed to be similar to that of other iridoviruses. Therefore, the RGV appears to be a member of the Iridoviridae based on these studies. (C) 1999 Elsevier Science B.V. All rights reserved. |
| 英文摘要 | A pathogenic virus (RGV), isolated from diseased pig frog Rana grylio with lethal syndrome, was investigated with regard to morphogenesis and cellular interactions in EPC cells, a cell Line from fish. Different stages of virus amplification, maturation and assembly were observed at nucleus, cytoplasm and cellular membranes. The matured virus particles, were not only distributed diffusely in nucleus, cytoplasm and cellular surface, but also aggregated as pseudocrystalline arrays in the cytoplasm. Virions were released by budding from the plasma membranes, or following cell lysis. Various types of cell damage, such as small vacuoles, spherical inclusions, and swollen and empty mitochondria, were also found. Some typical characteristics of RGV, such as the symmetrical shape of the virions, replication process involving both nuclear and cytoplasmic phases, budding release from cellular membrane and intracellular membrane, viromatrix and paracrystalline aggregation in cytoplasm, and its acute pathogenic effects, were observed to be similar to that of other iridoviruses. Therefore, the RGV appears to be a member of the Iridoviridae based on these studies. (C) 1999 Elsevier Science B.V. All rights reserved. |
| 学科主题 | Fisheries; Marine & Freshwater Biology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Marine & Freshwater Biology |
| 研究领域[WOS] | Fisheries ; Marine & Freshwater Biology |
| 关键词[WOS] | EPIZOOTIC HEMATOPOIETIC NECROSIS ; POLYMERASE-CHAIN-REACTION ; SEA BREAM IRIDOVIRUS ; BOHLE IRIDOVIRUS ; SEQUENCE-ANALYSIS ; BUFO-MARINUS ; REACTION PCR ; DEOXYRIBOVIRUS ; ULTRASTRUCTURE ; REPLICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000080556400001 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/10192]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, QY,Li, ZQ,Gui, JF. Studies on morphogenesis and cellular interactions of Rana grylio virus in an infected fish cell line[J]. AQUACULTURE,1999,175(3-4):185-197. |
| APA | Zhang, QY,Li, ZQ,&Gui, JF.(1999).Studies on morphogenesis and cellular interactions of Rana grylio virus in an infected fish cell line.AQUACULTURE,175(3-4),185-197. |
| MLA | Zhang, QY,et al."Studies on morphogenesis and cellular interactions of Rana grylio virus in an infected fish cell line".AQUACULTURE 175.3-4(1999):185-197. |
入库方式： OAI收割
来源：水生生物研究所
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