UV-sensitive P compounds: Release mechanism, seasonal fluctuation and inhibitory effects on alkaline phosphatase activity in a shallow Chinese freshwater lake (Donghu Lake)
文献类型：期刊论文
| 作者 | Zhou Yiyong |
| 刊名 | HYDROBIOLOGIA
 |
| 出版日期 | 1996 |
| 卷号 | 335期号:1页码:55-62 |
| 关键词 | UV-sensitive P compounds alkaline phosphatase in lake water hydrogen peroxide hydroxyl radical |
| 通讯作者 | Zhou, YY, WUHAN UNIV,INST HYDROBIOL,WUHAN 430072,PEOPLES R CHINA |
| 中文摘要 | Filtrable phosphorus compounds in a shallow Chinese freshwater lake (Donghu Lake) were fractionated by Sephadex G-25 gel-filtration chromatography. Some portions of those compounds released soluble reactive phosphorus upon irradiation with low dose ultraviolet light. Catalase and a hydroxyl radical scavenger (mannitol) markedly prevented photosensitive phosphorus release. The observed effects may be explained by the action of oxidizing reagents such as hydroxyl radicals, produced in photochemical reactions between UV irradiation and humic substances in the water. There was a strong seasonality in UV-sensitive P (UVSP) release. Michaels constants (K-m) of total alkaline phosphatase in the lake water showed a direct positive relation to UVSP. Plot of K-m against the UVSP/phosphomonoester ratio reveals a strong relationship between the two variables. These results suggest that in some situations UVSP may be a competitive inhibitor of alkaline phosphatase activity in the lake. The competitive inhibition of fractionated UVSP on alkaline phosphatase reagent (Sigma) apparently supports this hypothesis. |
| 学科主题 | Marine & Freshwater Biology |
| 收录类别 | SCI |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/10280]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhou Yiyong. UV-sensitive P compounds: Release mechanism, seasonal fluctuation and inhibitory effects on alkaline phosphatase activity in a shallow Chinese freshwater lake (Donghu Lake)[J]. HYDROBIOLOGIA,1996,335(1):55-62. |
| APA | Zhou Yiyong.(1996).UV-sensitive P compounds: Release mechanism, seasonal fluctuation and inhibitory effects on alkaline phosphatase activity in a shallow Chinese freshwater lake (Donghu Lake).HYDROBIOLOGIA,335(1),55-62. |
| MLA | Zhou Yiyong."UV-sensitive P compounds: Release mechanism, seasonal fluctuation and inhibitory effects on alkaline phosphatase activity in a shallow Chinese freshwater lake (Donghu Lake)".HYDROBIOLOGIA 335.1(1996):55-62. |
入库方式： OAI收割
来源：水生生物研究所
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