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A sensitive solid-phase time-resolved fluorescence immunoassay apparatus (EI CONFERENCE)

文献类型：会议论文

作者Song K.-F.; Li J.-L.; Zhang W.-L.; Wang Y.-L.
出版日期2009
会议名称International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging, June 17, 2009 - June 19, 2009
会议地点Beijing, China
关键词In the device a He-Ne laser of flash frequency 1-20 Hz was adopted as exciting light source and three key technical problems have been solved successfully in order to enhance the detecting sensitivity and measuring stability of the device for time-resolved fluorimmunoassays(TRFIA) [1]. The first one is to design optimum exciting optical system so that the exciting light beam excite the sample most effectively. The second one is to have a project spectrum filter which can reduce the affection of the background light to the photomultiplier tube and also ensure influence of the stray light and mixed diffusion light to the sample fluorescence to the least the sample fluorescence through the integrating sphere and come to the grating monochromator The right wavelength will be chosed through changing the angle of incidence of the grating monochromator. The third one is to simulate the principle of sample averaging of BOXCAR averager. In the device SCM was used as primary controller and CPLD was used as timing controller. Through the preparation process signal-to-noise ratio(SNR) will be improved also adjust delay time ampling frequency and sampling number arbitrarily. By testing the sensitivity is 10-12mol/L(substance marked by Eu3+) examination repeat is &le2.5% examination linearity is from 10 -8mol/L to 10-12mol/L correlation coefficient is 99.98%(p&le0.01). The instrument is advanced for ultrasensitive detection of antigen and antibody and solve the tumor genetic variation the virus protein detection. 2009 SPIE.
收录类别EI
源URL[http://ir.ciomp.ac.cn/handle/181722/33750]  
专题长春光学精密机械与物理研究所_中科院长春光机所知识产出_会议论文
推荐引用方式
GB/T 7714		 Song K.-F.,Li J.-L.,Zhang W.-L.,et al. A sensitive solid-phase time-resolved fluorescence immunoassay apparatus (EI CONFERENCE)[C]. 见:International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging, June 17, 2009 - June 19, 2009. Beijing, China.

入库方式： OAI收割

来源：长春光学精密机械与物理研究所

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