A graphene oxide based biosensor for microcystins detection by fluorescence resonance energy transfer
文献类型：期刊论文
| 作者 | Shi Y ; Wu JZ ; Sun YJ ; Zhang Y ; Wen ZW ; Dai HC ; Wang HD ; Li Z |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2012 |
| 卷号 | 38期号:1页码:31-36 |
| 关键词 | LIQUID-CHROMATOGRAPHY PROTEIN PHOSPHATASES CYANOBACTERIA LR NANOPARTICLES IMMUNOSENSOR HEPATOTOXINS APTASENSOR AERUGINOSA PLATFORM |
| ISSN号 | 0956-5663 |
| 通讯作者 | li z |
| 中文摘要 | water safety is one of the most pervasive problems afflicting people throughout the world. microcystin, a hepatotoxin produced by cyanobacteria, poses a growing and serious threat of water safety. according to world health organization (who), the limit of content of microcystin-lr (mc-lr) in drinking water is as low as 1 mu g/l; it is thus necessary to explore a sensitive method for the trace detection of microcystins (mcs). based on the observation of gold nanoparticles (au nps) induced graphene oxide (go) fluorescence quenching, a reliable biosensor was developed here for microcystins detection. mcs could be attached on au nps through the interaction with single strand-dna (ss-dna) modified on au nps, which formed au-dna-mcs complexes. these mcs in the complexes could be immunologically recognized by the antibodies adsorbed on go sheets, as a result, au nps were close enough to quench the photoluminescence of go by the fluorescence resonance energy transfer (fret). the fluorescence intensity decreased with the increase of mcs as more au nps linked onto go surface. the limit of detection was 0.5 and 0.3 mu g/l for microcystin-lr and microcystin-rr (mc-rr), respectively, which satisfies the strictest standard of who. well defined results were also obtained in natural lake water and the specificity experiment. the antibody used here could recognize adda group, the conservative part of mcs, which allowed the biosensor to detect both single toxin and the total content of mcs existing in the water sample. crown copyright (c) 2012 published by elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000308455700005 |
| 公开日期 | 2013-04-23 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/47923]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Shi Y,Wu JZ,Sun YJ,et al. A graphene oxide based biosensor for microcystins detection by fluorescence resonance energy transfer[J]. biosensors & bioelectronics,2012,38(1):31-36. |
| APA | Shi Y.,Wu JZ.,Sun YJ.,Zhang Y.,Wen ZW.,...&Li Z.(2012).A graphene oxide based biosensor for microcystins detection by fluorescence resonance energy transfer.biosensors & bioelectronics,38(1),31-36. |
| MLA | Shi Y,et al."A graphene oxide based biosensor for microcystins detection by fluorescence resonance energy transfer".biosensors & bioelectronics 38.1(2012):31-36. |
入库方式： OAI收割
来源：长春应用化学研究所
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