In situ labeling and imaging of cellular protein via a bi-functional anticancer aptamer and its fluorescent ligand
文献类型：期刊论文
| 作者 | Ai J ; Li T ; Li BL ; Xu YH ; Li D ; Liu ZJ ; Wang EK |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2012 |
| 卷号 | 741页码:93-99 |
| 关键词 | RNA DNA NANOPARTICLES NUCLEOLIN BINDING SURFACE PROBE |
| ISSN号 | 0003-2670 |
| 通讯作者 | wang ek |
| 中文摘要 | in this article, we reported a novel approach for in situ labeling and imaging hela cancer cells utilizing a bifunctional aptamer (as1411) and its fluorescent ligand, protoporphyrin ix (ppix). in the presence of potassium ion, as1411 folded to g-quadruplex structure, binded fluorescent ligand (ppix) with fluorescent enhancement, and targeted the nucleolin overexpressed by cancer cells. consequently, bioimaging of cancer cells specifically were realized by laser scanning confocal microscope. the bioimaging strategy with as1411-ppix complex was capable to distinguish hela cancer cells from normal cells unambiguously, and fluorescence imaging of cancer cells was also realized in human serum. moreover, the bioimaging method was very facile, effective and need not any covalent modification. these results illustrated that the useful approach can provide a novel clue for bioimaging based on non-covalent bifunctional aptamer in clinic diagnosis. (c) 2012 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000307689800012 |
| 公开日期 | 2013-04-23 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/47952]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Ai J,Li T,Li BL,et al. In situ labeling and imaging of cellular protein via a bi-functional anticancer aptamer and its fluorescent ligand[J]. analytica chimica acta,2012,741:93-99. |
| APA | Ai J.,Li T.,Li BL.,Xu YH.,Li D.,...&Wang EK.(2012).In situ labeling and imaging of cellular protein via a bi-functional anticancer aptamer and its fluorescent ligand.analytica chimica acta,741,93-99. |
| MLA | Ai J,et al."In situ labeling and imaging of cellular protein via a bi-functional anticancer aptamer and its fluorescent ligand".analytica chimica acta 741(2012):93-99. |
入库方式： OAI收割
来源：长春应用化学研究所
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