Electrochemical Single-Molecule AFM of the Redox Metalloenzyme Copper Nitrite Reductase in Action
文献类型：期刊论文
| 作者 | Hao X ; Zhang JD ; Christensen HEM ; Wang HD ; Ulstrup J |
| 刊名 | chemphyschem
 |
| 出版日期 | 2012 |
| 卷号 | 13期号:12页码:2919-2924 |
| 关键词 | SCANNING-TUNNELING-MICROSCOPY PROTEIN CYTOCHROME C(4) INTRAMOLECULAR ELECTRON-TRANSFER FORCE SPECTROSCOPY PSEUDOMONAS-STUTZERI AU(111) ELECTRODES VOLTAMMETRY SURFACES ASSEMBLIES MONOLAYERS |
| ISSN号 | 1439-4235 |
| 通讯作者 | ulstrup j |
| 中文摘要 | we studied the electrochemical behavior of the redox metalloenzyme copper nitrite reductase (cnir, achromobacter xylosoxidans) immobilized on a au(111)-electrode surface modified by a self-assembled cysteamine molecular monolayer (sam) using a combination of cyclic voltammetry and electrochemically-controlled atomic force microscopy (in situ afm). the enzyme showed no voltammetric signals in the absence of nitrite substrate, whereas a strong reductive electrocatalytic signal appeared in the presence of nitrite. such a pattern is common in protein film and monolayer voltammetry and points to conformational changes in the enzyme upon substrate binding. binding thus either improves the enzyme/electrode contact, or opens intramolecular electron-transfer channels between the redox center for electron inlet (a type i copper center) and the catalytic site for nitrite reduction (a type ii copper center). the in situ afm data are at the level of the single cunir enzyme molecule. the voltammetric patterns were paralleled by a clear increase (swelling) of the molecular height when the electrochemical potential traversed the region from resting to the electrocatalytically active redox enzyme function in the presence of nitrite. no change in size was observed in the absence of nitrite over the same potential range. the enzyme size variation is suggested to offer clues to the broadly observed substrate triggering in metalloenzyme monolayer voltammetry. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000307841900009 |
| 公开日期 | 2013-04-23 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/47988]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Hao X,Zhang JD,Christensen HEM,et al. Electrochemical Single-Molecule AFM of the Redox Metalloenzyme Copper Nitrite Reductase in Action[J]. chemphyschem,2012,13(12):2919-2924. |
| APA | Hao X,Zhang JD,Christensen HEM,Wang HD,&Ulstrup J.(2012).Electrochemical Single-Molecule AFM of the Redox Metalloenzyme Copper Nitrite Reductase in Action.chemphyschem,13(12),2919-2924. |
| MLA | Hao X,et al."Electrochemical Single-Molecule AFM of the Redox Metalloenzyme Copper Nitrite Reductase in Action".chemphyschem 13.12(2012):2919-2924. |
入库方式： OAI收割
来源：长春应用化学研究所
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