Lanthanum silicate coated magnetic microspheres as a promising affinity material for phosphopeptide enrichment and identification
文献类型：期刊论文
| 作者 | Cheng G ; Liu YL ; Zhang JL ; Sun DH ; Ni JZ |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2012 |
| 卷号 | 404期号:3页码:763-770 |
| ISSN号 | 1618-2642 |
| 关键词 | SELECTIVE ENRICHMENT PROTEIN-PHOSPHORYLATION MASS-SPECTROMETRY PHOSPHATE PEPTIDES SHELL CORE NANOPARTICLES PROTEOMICS REMOVAL |
| 通讯作者 | ni jz |
| 中文摘要 | novel fe3o4@la (x) si (y) o-5 affinity microspheres consisting of a superparamagnetic fe3o4 core and an amorphous lanthanum silicate shell have been synthesized. the core-shell-structured fe3o4@la (x) si (y) o-5 microspheres, with a mean size of ca. 480 nm, had rough lanthanum silicate surfaces and displayed relatively strong magnetism (47.2 emu g(-1)). this novel affinity material can be used for selective capture, rapid magnetic separation, and part dephosphorylation (which plays an important role in identifying phosphopeptides in ms) of the phosphopeptides in a peptide mixture. its ability to selectively trap and magnetically isolate as well as label the phosphopeptides was evaluated using a standard phosphorylated protein (beta-casein) and a real sample (human serum). phosphopeptides and their corresponding label ions were detected for concentrations of beta-casein as low as 1 x 10(-9) m and in mixtures of beta-casein and bsa with molar ratios as low as 1:50. in addition, this affinity material, with its labeling properties, is superior to commercial tio2 beads in terms of interference from non-phosphopeptide molecules. these results reveal that the lanthanum silicate coated magnetic microspheres represent a promising affinity material for the rapid purification and recognition of phosphopeptides. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000307251900018 |
| 公开日期 | 2013-04-23 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48017]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Cheng G,Liu YL,Zhang JL,et al. Lanthanum silicate coated magnetic microspheres as a promising affinity material for phosphopeptide enrichment and identification[J]. analytical and bioanalytical chemistry,2012,404(3):763-770. |
| APA | Cheng G,Liu YL,Zhang JL,Sun DH,&Ni JZ.(2012).Lanthanum silicate coated magnetic microspheres as a promising affinity material for phosphopeptide enrichment and identification.analytical and bioanalytical chemistry,404(3),763-770. |
| MLA | Cheng G,et al."Lanthanum silicate coated magnetic microspheres as a promising affinity material for phosphopeptide enrichment and identification".analytical and bioanalytical chemistry 404.3(2012):763-770. |
入库方式： OAI收割
来源：长春应用化学研究所
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