Probing the Interaction of Cisplatin with Cytochrome c by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
文献类型：期刊论文
| 作者 | Zhang NB ; Du YG ; Cui M ; Xing JP ; Liu ZQ ; Liu SY |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2012 |
| 卷号 | 84期号:14页码:6206-6212 |
| 关键词 | PROTEIN-LIGAND INTERACTIONS ANTICANCER PLATINUM DRUGS COPPER CHAPERONE ATOX1 HYDROGEN-EXCHANGE TOP-DOWN CONFORMATIONAL DYNAMICS SERUM-PROTEINS BINDING-SITES COMPLEXES ESI |
| ISSN号 | 0003-2700 |
| 通讯作者 | cui m |
| 中文摘要 | as one of the most important platinum drugs, the interactions of cisplatin with proteins play very important roles in its anticancer action and side effects. here, the interaction of cisplatin with cytochrome c was investigated using fourier transform ion cyclotron resonance mass spectrometry (fticr ms). on the basis of the high-resolution data, cytochrome c-pt(nh3)cl was found to be the primary monoadduct. the platinated monoadducts were related to molar ratios of cytochrome c and cisplatin, and corresponding reaction pathways were proposed. multiple binding sites of cisplatin on cytochrome c were directly determined by fticr ms combined with trypsin digestion without liquid chromatography (lc) separation. four binding sites (met65, met80, his18, and his33) for cisplatin on cytochrome c were identified. moreover, hydrogen/deuterium exchange (h/dx) combined with fticr ms provides the sensitive method to insight the small conformation change of cytochrome c induced by cisplatin. this strategy can be applied to comprehensive investigation of metallodrug/protein complexes. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000306441200053 |
| 公开日期 | 2013-05-20 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48040]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang NB,Du YG,Cui M,et al. Probing the Interaction of Cisplatin with Cytochrome c by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry[J]. analytical chemistry,2012,84(14):6206-6212. |
| APA | Zhang NB,Du YG,Cui M,Xing JP,Liu ZQ,&Liu SY.(2012).Probing the Interaction of Cisplatin with Cytochrome c by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.analytical chemistry,84(14),6206-6212. |
| MLA | Zhang NB,et al."Probing the Interaction of Cisplatin with Cytochrome c by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry".analytical chemistry 84.14(2012):6206-6212. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。