Studies of interaction between insulin and glutathione using electrospray ionization mass spectrometry
文献类型：期刊论文
| 作者 | Du YG ; Zhang NB ; Cui M ; Liu ZQ ; Liu SY |
| 刊名 | rapid communications in mass spectrometry
 |
| 出版日期 | 2012 |
| 卷号 | 26期号:13页码:1519-1526 |
| 关键词 | ENZYMATIC REDUCTION DISULPHIDE BONDS REDOX PROTEOMICS SERUM ALBUMIN PROTEIN IDENTIFICATION RIBONUCLEASE DISULFIDES REACTIVITY CONSTANTS |
| ISSN号 | 0951-4198 |
| 通讯作者 | cui m |
| 中文摘要 | rationale the interaction of glutathione (gsh) with insulin plays an important role in the degradation or regulation of insulin. the characterization of the reaction products of gsh and insulin is very important for a proper understanding of the mechanism of insulin regulation of gsh. methods solutions of insulin and glutathione were incubated under different experimental conditions in vitro. the reaction products were determined by electrospray ionization (esi) ion trap mass spectrometry combined with fourier transform ion cyclotron resonance (fticr) mass spectrometry. results the multi-reaction products were identified, including insulin a chain with two intrachain disulfides, insulin b chain with one intrachain disulfides, gsh-modified insulin, gsh-modified a chain, gsh-modified b chain, aggregates of a chain and b chain, and reduced a chain and b chain. the binding site of the b chain with insulin was determined directly by tandem mass spectrometry (ms/ms) without enzyme digestion. it was found that the reaction between gsh and insulin was ph-, o2- and temperature-dependent. conclusions the results provide insight into the interaction between gsh and insulin. it has also been demonstrated that esi-ms combined with high-resolution fticrms and ms/ms provides a powerful tool for screening the reactions of proteins and small molecules. copyright (c) 2012 john wiley & sons, ltd. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000304438500001 |
| 公开日期 | 2013-05-20 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48050]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Du YG,Zhang NB,Cui M,et al. Studies of interaction between insulin and glutathione using electrospray ionization mass spectrometry[J]. rapid communications in mass spectrometry,2012,26(13):1519-1526. |
| APA | Du YG,Zhang NB,Cui M,Liu ZQ,&Liu SY.(2012).Studies of interaction between insulin and glutathione using electrospray ionization mass spectrometry.rapid communications in mass spectrometry,26(13),1519-1526. |
| MLA | Du YG,et al."Studies of interaction between insulin and glutathione using electrospray ionization mass spectrometry".rapid communications in mass spectrometry 26.13(2012):1519-1526. |
入库方式： OAI收割
来源：长春应用化学研究所
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