Structural characterization and immunological activities of the water-soluble oligosaccharides isolated from the Panax ginseng roots
文献类型:期刊论文
作者 | Wan DB ; Jiao LL ; Yang HM ; Liu SY |
刊名 | planta
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出版日期 | 2012 |
卷号 | 235期号:6页码:1289-1297 |
关键词 | ACTIVE ANTIRETROVIRAL THERAPY TANDEM MASS-SPECTROMETRY KOREAN RED GINSENG POLYSACCHARIDES SAPONIN ASSAY |
ISSN号 | 0032-0935 |
通讯作者 | liu sy |
中文摘要 | water-soluble ginseng oligosaccharides (designated as wgos) with a degree of polymerization ranging from 2 to 10 were obtained from warm-water extract of panax ginseng roots, and fractionated into five purified fractions (i.e., wgos-0, wgos-1, wgos-2, wgos-3, and wgos-4) by gel-filtration chromatography. in order to ascertain the monosaccharide residues in the wgos, a technique that combines acid hydrolysis and high-performance liquid chromatography was employed. it was found that only glucose residues were present in the wgos. fourier transform infrared spectroscopy and electrospray ionization tandem mass spectrometry provided the sequence, linkage, and configuration information. it is noteworthy that alpha-glcp-(1 -> 6)-alpha-glcp, alpha-glcp-(1 -> 6)-alpha-glcp-(1 -> 4)-alpha-glcp, alpha-glcp-(1 -> 6)-alpha-glcp-(1 -> 6)-alpha-glcp-(1 -> 4)-alpha-glcp, and other six malto-oligosaccharides (i.e., maltopentaose, maltohexaose, maltoheptaose, maltooctaose, maltononaose, and maltodecaose) were detected in ginseng. preliminary immunological tests in vitro indicated that wgos were potent b and t-cell stimulators and wgos-1 has the highest immunostimulating effect on lymphocyte proliferation among those purified fractions. it is hoped that the wgos will be developed into functional food or medicine. |
收录类别 | SCI收录期刊论文 |
语种 | 英语 |
WOS记录号 | WOS:000304621200014 |
公开日期 | 2013-05-20 |
源URL | [http://ir.ciac.jl.cn/handle/322003/48122] ![]() |
专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
推荐引用方式 GB/T 7714 | Wan DB,Jiao LL,Yang HM,et al. Structural characterization and immunological activities of the water-soluble oligosaccharides isolated from the Panax ginseng roots[J]. planta,2012,235(6):1289-1297. |
APA | Wan DB,Jiao LL,Yang HM,&Liu SY.(2012).Structural characterization and immunological activities of the water-soluble oligosaccharides isolated from the Panax ginseng roots.planta,235(6),1289-1297. |
MLA | Wan DB,et al."Structural characterization and immunological activities of the water-soluble oligosaccharides isolated from the Panax ginseng roots".planta 235.6(2012):1289-1297. |
入库方式: OAI收割
来源:长春应用化学研究所
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