Enantioselective and label-free detection of oligopeptide via fluorescent indicator displacement
文献类型：期刊论文
| 作者 | Ren JT ; Wang JH ; Wang J ; Luedtke NW ; Wang EK |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2012 |
| 卷号 | 35期号:1页码:401-406 |
| 关键词 | L-RNA APTAMER CHIRAL STATIONARY-PHASE CAPILLARY-ELECTROPHORESIS HIV-1 ENTRY AMINO-ACIDS TARGET ENANTIOMERS SEPARATION PHTHALOCYANINES RECOGNITION |
| ISSN号 | 0956-5663 |
| 通讯作者 | wang jh |
| 中文摘要 | in this work, a simple and label-free fluorescent method via fluorescent indicator displacement (fid) was proposed for enantioselectively determining d-enantiomer of arginine vasopressin (dv) using dv-specific dna aptamer (v-apt) and one guanidiniophthalocyanine dye (zn-digp). zn-digp that preferentially binds to single-stranded dna with fluorescence enhancement rather than duplexes occupies the long internal loop of v-apt and generates intensive fluorescence. then dv is introduced into the solution containing zn-digp and v-apt, and displaces the zn-digp from the binding site of internal loop, leading to fluorescence decrease. but l-enantiomer cannot induce any fluorescence change due to the selectivity of v-apt. this established fid technique can detect dv with a detection limit of 100 nm and exhibits a broad linear range, and is able to discriminate enantiomers of arginine vasopressin unambiguously. moreover chiral separation by chromatography, complicated experimental procedures and covalent modification of tags (such as organic dyes, redox-active metal complexes) are avoided in our strategy. this simple and label-free method is promising for fabricating diverse aptasensors to determine other biomolecules and drugs. (c) 2012 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000305036000061 |
| 公开日期 | 2013-05-20 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48159]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Ren JT,Wang JH,Wang J,et al. Enantioselective and label-free detection of oligopeptide via fluorescent indicator displacement[J]. biosensors & bioelectronics,2012,35(1):401-406. |
| APA | Ren JT,Wang JH,Wang J,Luedtke NW,&Wang EK.(2012).Enantioselective and label-free detection of oligopeptide via fluorescent indicator displacement.biosensors & bioelectronics,35(1),401-406. |
| MLA | Ren JT,et al."Enantioselective and label-free detection of oligopeptide via fluorescent indicator displacement".biosensors & bioelectronics 35.1(2012):401-406. |
入库方式： OAI收割
来源：长春应用化学研究所
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