Pd Nanowires as New Biosensing Materials for Magnified Fluorescent Detection of Nucleic Add
文献类型：期刊论文
| 作者 | Zhang LB ; Guo SJ ; Dong SJ ; Wang EK |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2012 |
| 卷号 | 84期号:8页码:3568-3573 |
| 关键词 | CARBON NANOTUBES NANOPARTICLE PROBES MOLECULAR BEACONS SENSING PLATFORM GENE-REGULATION DNA DELIVERY OLIGONUCLEOTIDES HYBRIDIZATION BIOMOLECULES |
| ISSN号 | 0003-2700 |
| 通讯作者 | wang ek |
| 中文摘要 | the designed synthesis of new nanomaterials with controlled shape, composition, and structure is critical for tuning their physical and chemical properties, and further developing interesting analytical sensing devices. herein, we presented that pd nanowires (nws) can be used as a new biosensing platform for high-sensitivity nucleic acid detection. the general sensing concept is based on the fact that pd nws can adsorb the fluorescently labeled single-stranded dna probe and lead to substantial fluorescence quenching of dye, followed by specific hybridization with the complementary region of the target dna sequence. this results in desorption of double-stranded dna from pd nws surface and subsequent recovery of fluorescence. furthermore, an amplification strategy based on pd nws for nucleic acid detection by using exonuclease iii (exo iii) was demonstrated. the present dual-magnification sensing system combined pd nws with exo iii has a detection range of 1.0 nm to 2.0 mu m with the detection limit of 0.3 nm (s/n = 3), which is about 20-fold higher than that of traditional unamplified homogeneous assays. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000302838500013 |
| 公开日期 | 2013-05-22 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48212]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang LB,Guo SJ,Dong SJ,et al. Pd Nanowires as New Biosensing Materials for Magnified Fluorescent Detection of Nucleic Add[J]. analytical chemistry,2012,84(8):3568-3573. |
| APA | Zhang LB,Guo SJ,Dong SJ,&Wang EK.(2012).Pd Nanowires as New Biosensing Materials for Magnified Fluorescent Detection of Nucleic Add.analytical chemistry,84(8),3568-3573. |
| MLA | Zhang LB,et al."Pd Nanowires as New Biosensing Materials for Magnified Fluorescent Detection of Nucleic Add".analytical chemistry 84.8(2012):3568-3573. |
入库方式： OAI收割
来源：长春应用化学研究所
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