An ultrasensitive fluorescent aptasensor for adenosine detection based on exonuclease III assisted signal amplification
文献类型：期刊论文
| 作者 | Hu P ; Zhu CZ ; Jin LH ; Dong SJ |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2012 |
| 卷号 | 34期号:1页码:83-87 |
| 关键词 | ESCHERICHIA-COLI ELECTROCHEMICAL BIOSENSOR DEOXYRIBONUCLEIC ACID QUANTUM DOTS IN-VIVO DNA APTAMER ASSAY MOLECULES DIGESTION |
| ISSN号 | 0956-5663 |
| 通讯作者 | dong sj |
| 中文摘要 | we report here a graphene oxide (go)-based fluorescent aptasensor for adenosine detection by employing exonuclease iii (exo iii) as a signal amplifying element. in the absence of adenosine, the adenosine aptamers hybridized with the complementary dna (cdna), and the exo iii could not cleave the single-strand signal probes labeled with carboxylfluorescein (fam) at its 5' ends. when the graphene oxide was finally added, it could strongly adsorb the single-strand signal probes and quenched the fluorophore effectively. in the presence of adenosine, the aptamers associated with the targets, which led to the formation of duplex dnas between the cdnas and the signal probes. the exo iii thereafter could digest the duplex dnas from 3' blunt terminus of signal probes, liberating the fluorophore. upon adding the go, the fluorophore could not be adsorbed and quenched. by coupling cyclic enzymatic cleavage, a remarkable fluorescent increase was obtained. due to the specific recognition ability of the aptamer for the target and the powerful quenching property of go for signal probe, this proposed approach has a good selectivity and high sensitivity for adenosine. in the optimum conditions described, >100% signal enhancement was achieved and a limit of detection as low as 1 nm was obtained, which is lower than those of commonly used fluorescent aptamer sensors. moreover, the biosensor exhibited an ultrahigh sensitivity and held a versatile platform for clinical diagnostics, molecular biology and drug developments. (c) 2012 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000302886500012 |
| 公开日期 | 2013-05-22 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48222]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Hu P,Zhu CZ,Jin LH,et al. An ultrasensitive fluorescent aptasensor for adenosine detection based on exonuclease III assisted signal amplification[J]. biosensors & bioelectronics,2012,34(1):83-87. |
| APA | Hu P,Zhu CZ,Jin LH,&Dong SJ.(2012).An ultrasensitive fluorescent aptasensor for adenosine detection based on exonuclease III assisted signal amplification.biosensors & bioelectronics,34(1),83-87. |
| MLA | Hu P,et al."An ultrasensitive fluorescent aptasensor for adenosine detection based on exonuclease III assisted signal amplification".biosensors & bioelectronics 34.1(2012):83-87. |
入库方式： OAI收割
来源：长春应用化学研究所
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