Theoretical Analysis of Catalytic-sRNA-Mediated Gene Silencing
文献类型：期刊论文
| 作者 | Shi, HL
|
| 刊名 | JOURNAL OF MOLECULAR BIOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 406期号:1页码:195-204 |
| 关键词 | TRANSCRIPTIONAL REGULATION ESCHERICHIA-COLI REGULATORY RNAS BACTERIA EXPRESSION NOISE CONSEQUENCES NUMBERS STRESS SYSTEM |
| ISSN号 | 0022-2836 |
| 通讯作者 | Shi, HL (reprint author), Chinese Acad Sci, Inst Theoret Phys, Beijing 100190, Peoples R China. |
| 英文摘要 | Small regulatory RNA (sRNA) that acts by an antisense mechanism is critical for gene regulation at the posttranscriptional level. Recently, an Hfq-dependent sRNA named MicM, which is related to the regulation of outer membrane protein, was verified as a novel antisense sRNA due to its catalytic mode of regulation. Here we propose a simple kinetic model for the enzyme-like regulation mode of sRNA and study in detail the noise properties of the target gene under various recycling rates of the regulator. We predict that the recycling rate of sRNA and other relative parameters have significant influence on the noise strength of target expression. In comparison with the stoichiometric regulatory mode, a lesser fluctuation of target expression was observed near the threshold at which the transcription rates of both sRNA and target mRNA equal each other. We also found that the new mode is better in terms of rapid response to external signals. However, it needs more time to achieve target recovery if the stimulating signal disappears. Additionally, the obtained time evolution results of the MicM-ybfM interaction system based on our model are consistent with previous experimental results, serving as experimental evidence to back up our theoretical analysis. (C) 2010 Elsevier Ltd. All rights reserved. |
| 学科主题 | Physics |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China [2007CB814800] |
| 原文出处 | http://dx.doi.org/10.1016/j.jmb.2010.12.008 |
| 语种 | 英语 |
| WOS记录号 | WOS:000287281800015 |
| 公开日期 | 2013-05-17 |
| 源URL | [http://ir.itp.ac.cn/handle/311006/14411]  |
| 专题 | 理论物理研究所_理论物理所1978-2010年知识产出 |
| 推荐引用方式 GB/T 7714 | Shi, HL. Theoretical Analysis of Catalytic-sRNA-Mediated Gene Silencing[J]. JOURNAL OF MOLECULAR BIOLOGY,2011,406(1):195-204. |
| APA | Shi, HL.(2011).Theoretical Analysis of Catalytic-sRNA-Mediated Gene Silencing.JOURNAL OF MOLECULAR BIOLOGY,406(1),195-204. |
| MLA | Shi, HL."Theoretical Analysis of Catalytic-sRNA-Mediated Gene Silencing".JOURNAL OF MOLECULAR BIOLOGY 406.1(2011):195-204. |
入库方式： OAI收割
来源：理论物理研究所
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