Forward transport of proteins in the plasma membrane of migrating cerebellar granule cells
文献类型：期刊论文
| 作者 | Poo, MM
|
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| 出版日期 | 2012 |
| 卷号 | 109期号:51页码:E3558-E3567 |
| ISSN号 | 0027-8424 |
| 关键词 | NEURONAL GROWTH CONES F-ACTIN FLOW CYTOSKELETAL DYNAMICS CULTURED HIPPOCAMPAL LATERAL DIFFUSION CORTICAL FLOW NERVE GROWTH ANIMAL-CELLS FIBROBLASTS SURFACE |
| 通讯作者 | Poo, MM (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Neurosci, Inst Neurosci, Shanghai 200031, Peoples R China.,mpoo@berkeley.edu |
| 英文摘要 | Directional flow of membrane components has been detected at the leading front of fibroblasts and the growth cone of neuronal processes, but whether there exists global directional flow of plasma membrane components over the entire migrating neuron remains largely unknown. By analyzing the trajectories of antibody-coated single quantum dots (QDs) bound to two membrane proteins, overexpressed myc-tagged synaptic vesicle-associated membrane protein VAMP2 and endogenous neurotrophin receptor TrkB, we found that these two proteins exhibited net forward transport, which is superimposed upon Brownian motion, in both leading and trailing processes of migrating cerebellar granule cells in culture. Furthermore, no net directional transport of membrane proteins was observed in nonmigrating cells with either growing or stalling leading processes. Analysis of the correlation of motion direction between two QDs on the same process in migrating neurons also showed a higher frequency of correlated forward than rearward movements. Such correlated QD movements were markedly reduced in the presence of myosin II inhibitor blebbistatin, suggesting the involvement of myosin II-dependent active transport processes. Thus, a net forward transport of plasma membrane proteins exists in the leading and trailing processes of migrating neurons, in line with the translocation of the soma. |
| 学科主题 | Science & Technology - Other Topics |
| 资助信息 | National Basic Research Program of China [2011CBA00400] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-06-04 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/2482]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Poo, MM. Forward transport of proteins in the plasma membrane of migrating cerebellar granule cells[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2012,109(51):E3558-E3567. |
| APA | Poo, MM.(2012).Forward transport of proteins in the plasma membrane of migrating cerebellar granule cells.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,109(51),E3558-E3567. |
| MLA | Poo, MM."Forward transport of proteins in the plasma membrane of migrating cerebellar granule cells".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 109.51(2012):E3558-E3567. |
入库方式： OAI收割
来源：上海神经科学研究所
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