Microglial migration mediated by ATP-induced ATP release from lysosomes
文献类型：期刊论文
| 作者 | Dou, Y ; Wu, HJ ; Li, HQ ; Qin, S ; Wang, YE ; Li, J ; Lou, HF ; Chen, Z ; Li, XM ; Luo, QM ; Duan, SM |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2012 |
| 卷号 | 22期号:6页码:1022-1033 |
| ISSN号 | 1001-0602 |
| 关键词 | CA2+-REGULATED EXOCYTOSIS SECRETORY LYSOSOMES IN-VIVO ASTROCYTES CELLS RECEPTORS MEMBRANE FLUORESCENCE NUCLEOTIDES ACTIVATION |
| 通讯作者 | Duan, SM (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, Shanghai 200031, Peoples R China.,duanshumin@zju.edu.cn |
| 英文摘要 | Microglia are highly motile cells that act as the main form of active immune defense in the central nervous system. Attracted by factors released from damaged cells, microglia are recruited towards the damaged or infected site, where they are involved in degenerative and regenerative responses and phagocytotic clearance of cell debris. ATP release from damaged neural tissues has been suggested to mediate the rapid extension of microglial process towards the site of injury. However, the mechanisms of the long-range migration of microglia remain to be clarified. Here, we found that lysosomes in microglia contain abundant ATP and exhibit Ca2+-dependent exocytosis in response to various stimuli. By establishing an efficient in vitro chemotaxis assay, we demonstrated that endogenously-released ATP from microglia triggered by local microinjection of ATP gamma S is critical for the long-range chemotaxis of microglia, a response that was significantly inhibited in microglia treated with an agent inducing lysosome osmodialysis or in cells derived from mice deficient in Rab 27a (ashen mice), a small GTPase required for the trafficking and exocytosis of secretory lysosomes. These results suggest that microglia respond to extracellular ATP by releasing ATP themselves through lysosomal exocytosis, thereby providing a positive feedback mechanism to generate a long-range extracellular signal for attracting distant microglia to migrate towards and accumulate at the site of injury. |
| 学科主题 | Cell Biology |
| 资助信息 | Major State Basic Research Program of China [2011CB504400, 2011CBA00400]; National Natural Science Foundation of China [30730037, 30800321] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2013-06-04 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/2489]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Dou, Y,Wu, HJ,Li, HQ,et al. Microglial migration mediated by ATP-induced ATP release from lysosomes[J]. CELL RESEARCH,2012,22(6):1022-1033. |
| APA | Dou, Y.,Wu, HJ.,Li, HQ.,Qin, S.,Wang, YE.,...&Duan, SM.(2012).Microglial migration mediated by ATP-induced ATP release from lysosomes.CELL RESEARCH,22(6),1022-1033. |
| MLA | Dou, Y,et al."Microglial migration mediated by ATP-induced ATP release from lysosomes".CELL RESEARCH 22.6(2012):1022-1033. |
入库方式： OAI收割
来源：上海神经科学研究所
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