Robo4 Regulates the Radial Migration of Newborn Neurons in Developing Neocortex
文献类型：期刊论文
| 作者 | Zheng, W ; Geng, AQ ; Li, PF ; Wang, Y ; Yuan, XB |
| 刊名 | CEREBRAL CORTEX
 |
| 出版日期 | 2012 |
| 卷号 | 22期号:11页码:2587-2601 |
| 关键词 | AXON GUIDANCE RECEPTORS CELL-MIGRATION CEREBRAL-CORTEX IN-VIVO MAGIC ROUNDABOUT GROWTH CONE SLIT ANGIOGENESIS MIDLINE MECHANISMS |
| ISSN号 | 1047-3211 |
| 通讯作者 | Yuan, XB (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, Shanghai 200031, Peoples R China.,yuanxb@ion.ac.cn |
| 英文摘要 | During the morphogenesis of neocortex, newborn neurons undergo radial migration from the ventricular zone toward the surface of the cortical plate to form an "inside-out" lamina structure. The spatiotemporal signals that control this stereotyped radial migration remain elusive. Here, we report that a recently identified Robo family member Robo4 (Magic Roundabout), which was considered to be solely expressed in endothelial cells, is expressed in developing brain and regulates the radial migration of newborn neurons in neocortex. Downregulation of Robo4 expression in cortical newborn neurons by using in utero electroporation, with either specific siRNAs in wild-type rodents or with Cre recombinase in floxed-robo4 mutant mice, led to severe defects in the radial migration of newborn neurons with misorientation of these neurons. Moreover, newborn neurons transfected with Robo4 siRNAs exhibited significantly lower motility in a transwell migration assay (Boyden chamber) in the absence of Slit and significantly higher sensitivity to the repulsive effect of Slit in both transwell migration assay and growth cone collapse assay. Overall, our results showed an important role of Robo4 in the regulation of cortical radial migration through Slit-dependent and -independent mechanisms. |
| 学科主题 | Neurosciences & Neurology |
| 收录类别 | SCI |
| 资助信息 | 973 project [2011CBA00400]; National Natural Science Foundation of China [31021063]; SA-SIBS Scholarship |
| 语种 | 英语 |
| 公开日期 | 2013-06-04 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/2499]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Zheng, W,Geng, AQ,Li, PF,et al. Robo4 Regulates the Radial Migration of Newborn Neurons in Developing Neocortex[J]. CEREBRAL CORTEX,2012,22(11):2587-2601. |
| APA | Zheng, W,Geng, AQ,Li, PF,Wang, Y,&Yuan, XB.(2012).Robo4 Regulates the Radial Migration of Newborn Neurons in Developing Neocortex.CEREBRAL CORTEX,22(11),2587-2601. |
| MLA | Zheng, W,et al."Robo4 Regulates the Radial Migration of Newborn Neurons in Developing Neocortex".CEREBRAL CORTEX 22.11(2012):2587-2601. |
入库方式： OAI收割
来源：上海神经科学研究所
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