A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins
文献类型：期刊论文
| 作者 | Lukinavicius, G ; Umezawa, K ; Olivier, N ; Honigmann, A ; Yang, GY ; Plass, T ; Mueller, V ; Reymond, L ; Correa, IR ; Luo, ZG ; Schultz, C ; Lemke, EA ; Heppenstall, P ; Eggeling, C ; Manley, S ; Johnsson, K |
| 刊名 | NATURE CHEMISTRY
 |
| 出版日期 | 2013 |
| 卷号 | 5期号:2页码:132-139 |
| 关键词 | DIELS-ALDER REACTIONS LIVING CELLS FUSION PROTEINS FAR-RED FLUORESCENCE MICROSCOPY SYNTHETIC FLUOROPHORES STIMULATED-EMISSION STED NANOSCOPY SI-RHODAMINE MOUSE-BRAIN |
| ISSN号 | 1755-4330 |
| 通讯作者 | Lukinavicius, G (reprint author), Ecole Polytech Fed Lausanne, Inst Chem Sci & Engn ISIC, Inst Bioengn, Natl Ctr Competence Res NCCR Chem Biol, CH-1015 Lausanne, Switzerland.,kai.johnsson@epfl.ch |
| 英文摘要 | The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can be implemented flexibly in living cells and in vivo. However, the design of synthetic fluorophores that combine all of these properties has proved to be extremely difficult. Here, we introduce a biocompatible near-infrared silicon-rhodamine probe that can be coupled specifically to proteins using different labelling techniques. Importantly, its high permeability and fluorogenic character permit the imaging of proteins in living cells and tissues, and its brightness and photostability make it ideally suited for live-cell super-resolution microscopy. The excellent spectroscopic properties of the probe combined with its ease of use in live-cell applications make it a powerful new tool for bioimaging. |
| 学科主题 | Chemistry |
| 收录类别 | SCI |
| 资助信息 | Swiss National Science Foundation; Chemical National Centre of Competence in Research Biology, European Research Council [243016]; Ecole Polytechnique Federale de Lausanne; Federation of European Biochemical Societies long-term fellowship; Fonds der Chemischen Industrie; Emmy Noether program of the Deutsche Forschungsgemeinschaft; Deutsche Forschungsgemeinschaft [SPP 1623]; [TRR83] |
| 语种 | 英语 |
| 公开日期 | 2013-06-04 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/2512]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Lukinavicius, G,Umezawa, K,Olivier, N,et al. A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins[J]. NATURE CHEMISTRY,2013,5(2):132-139. |
| APA | Lukinavicius, G.,Umezawa, K.,Olivier, N.,Honigmann, A.,Yang, GY.,...&Johnsson, K.(2013).A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins.NATURE CHEMISTRY,5(2),132-139. |
| MLA | Lukinavicius, G,et al."A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins".NATURE CHEMISTRY 5.2(2013):132-139. |
入库方式： OAI收割
来源：上海神经科学研究所
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