Novel Use of Poly(3,4-ethylenedioxythiophene) Nanoparticles for Fluorescent Nucleic Acid Detection
文献类型：期刊论文
| 作者 | Zhang YW ; Liu S ; Wang L ; Luo YL ; Tian JQ ; Asiri AM ; Al-Youbi AO ; Sun XP |
| 刊名 | acs combinatorial science
 |
| 出版日期 | 2012 |
| 卷号 | 14期号:3页码:191-196 |
| 关键词 | EFFECTIVE SENSING PLATFORM CARBON NANOTUBES DNA DETECTION PROBES GRAPHENE OLIGONUCLEOTIDES BIOMOLECULES |
| ISSN号 | 2156-8952 |
| 通讯作者 | sun xp |
| 中文摘要 | in this paper, we demonstrate the novel use of poly(3,4-ethylene dioxythiophene) (pedot) nanoparticle as a very effective fluorescent sensing platform for the detection of nucleic acid sequences. the principle of the assay lies in the fact that the adsorption of the fluorescently labeled single-stranded dna (ssdna) probe by pedot nanoparticle leads to substantial fluorescence quenching, followed by specific hybridization with the complementary region of the target dna sequence. this results in desorption of the hybridized complex from pedot nanoparticle surface and subsequent recovery of fluorescence. a detection limit as low as 30 pm could be achieved in this sensing system. we also demonstrate its application for multiplexed detection of nucleic acid sequences. furthermore, this sensing system can realize the detection of single-base mismatch even in multiplexed format. it is of importance to note that the successful use of this sensing platform in human blood serum system is also demonstrated. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000301310600008 |
| 公开日期 | 2013-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48292]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhang YW,Liu S,Wang L,et al. Novel Use of Poly(3,4-ethylenedioxythiophene) Nanoparticles for Fluorescent Nucleic Acid Detection[J]. acs combinatorial science,2012,14(3):191-196. |
| APA | Zhang YW.,Liu S.,Wang L.,Luo YL.,Tian JQ.,...&Sun XP.(2012).Novel Use of Poly(3,4-ethylenedioxythiophene) Nanoparticles for Fluorescent Nucleic Acid Detection.acs combinatorial science,14(3),191-196. |
| MLA | Zhang YW,et al."Novel Use of Poly(3,4-ethylenedioxythiophene) Nanoparticles for Fluorescent Nucleic Acid Detection".acs combinatorial science 14.3(2012):191-196. |
入库方式： OAI收割
来源：长春应用化学研究所
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