Displacement immunoassay for the detection of ochratoxin A using ochratoxin B modified glass beads
文献类型：期刊论文
| 作者 | Lates V ; Yang C ; Popescu IC ; Marty JL |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2012 |
| 卷号 | 402期号:9页码:2861-2870 |
| 关键词 | LIQUID-CHROMATOGRAPHIC ANALYSIS IMMUNOAFFINITY COLUMN CLEANUP IMMUNOSENSOR QUANTIFICATION SPECTROMETRY ANTIBODIES SAMPLES SERUM WINES DNA |
| ISSN号 | 1618-2642 |
| 通讯作者 | marty jl |
| 中文摘要 | we report here the development of a new assay for the detection of ochratoxin a (ota) based on the use of its dechlorinated analogue, ochratoxin b (otb), in a displacement immunoassay. otb was immobilised on controlled-pore glass beads followed by the binding of anti-ota antibody, with anti-igg antibody peroxidase conjugate used as a label. in this manner, an original bio-sensing material was obtained. upon incubation of this material with ota, the toxin competes with otb for the binding sites of the anti-ota antibodies and releases the antibody-tagged peroxidase complex into the solution. compared to classic competitive immunoassays, this newly developed displacement immunoassay presents a similar detection limit and assay time. moreover, the detection, based on the activity of the horseradish peroxidase, is performed for the first time in situ using wine samples. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000301178000017 |
| 公开日期 | 2013-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48297]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Lates V,Yang C,Popescu IC,et al. Displacement immunoassay for the detection of ochratoxin A using ochratoxin B modified glass beads[J]. analytical and bioanalytical chemistry,2012,402(9):2861-2870. |
| APA | Lates V,Yang C,Popescu IC,&Marty JL.(2012).Displacement immunoassay for the detection of ochratoxin A using ochratoxin B modified glass beads.analytical and bioanalytical chemistry,402(9),2861-2870. |
| MLA | Lates V,et al."Displacement immunoassay for the detection of ochratoxin A using ochratoxin B modified glass beads".analytical and bioanalytical chemistry 402.9(2012):2861-2870. |
入库方式： OAI收割
来源：长春应用化学研究所
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