Ultrahigh performance liquid chromatography-triple quadrupole mass spectrometry inhibitors fishing assay: A novel method for simultaneously screening of xanthine oxidase inhibitor and superoxide anion scavenger in a single analysis
文献类型：期刊论文
| 作者 | Liu S ; Xing JP ; Zheng Z ; Song FR ; Liu ZQ ; Liu SY |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2012 |
| 卷号 | 715页码:64-70 |
| 关键词 | NON-PURINE OXIDASE/XANTHINE DEHYDROGENASE ANTIOXIDANT ACTIVITY SELECTIVE INHIBITOR FEBUXOSTAT TMX-67 FLAVONOIDS GOUT HYPERURICEMIA ALLOPURINOL Y-700 |
| ISSN号 | 0003-2670 |
| 通讯作者 | liu zq |
| 中文摘要 | xanthine oxidase (xod) inhibitors and superoxide anion scavengers play an important role in the treatment of gout and the inhibition of many diseases related to superoxide anion. the respective quantitation of uric acid and superoxide anion by traditional spectroscopic methods is routine in xod inhibitors and superoxide anion scavengers screening at laboratories worldwide. in the present study, we established an ultrahigh performance liquid chromatography and triple quadrupole mass spectrometry (uhplc-tq-ms) method of higher accuracy and speed that combines screening of superoxide anion scavenger and xod inhibitor in a single analysis by adding wst-1 (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2h-tetrazolium sodium salt) to the enzymatic reaction. we applied the established method to determine the xod inhibitory activities and superoxide scavenging activities of some herbal extracts and compounds from natural products, which could be classified into six groups based on the results of the assay. our innovative protocol is fast, accurate and robust. moreover, it can eliminate false positive and false negative results which may occur in the traditional spectroscopic methods. (c) 2011 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000300134200006 |
| 公开日期 | 2013-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48316]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu S,Xing JP,Zheng Z,et al. Ultrahigh performance liquid chromatography-triple quadrupole mass spectrometry inhibitors fishing assay: A novel method for simultaneously screening of xanthine oxidase inhibitor and superoxide anion scavenger in a single analysis[J]. analytica chimica acta,2012,715:64-70. |
| APA | Liu S,Xing JP,Zheng Z,Song FR,Liu ZQ,&Liu SY.(2012).Ultrahigh performance liquid chromatography-triple quadrupole mass spectrometry inhibitors fishing assay: A novel method for simultaneously screening of xanthine oxidase inhibitor and superoxide anion scavenger in a single analysis.analytica chimica acta,715,64-70. |
| MLA | Liu S,et al."Ultrahigh performance liquid chromatography-triple quadrupole mass spectrometry inhibitors fishing assay: A novel method for simultaneously screening of xanthine oxidase inhibitor and superoxide anion scavenger in a single analysis".analytica chimica acta 715(2012):64-70. |
入库方式： OAI收割
来源：长春应用化学研究所
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