Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A
文献类型：期刊论文
| 作者 | Yang C ; Lates V ; Prieto-Simon B ; Marty JL ; Yang XR |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2012 |
| 卷号 | 32期号:1页码:208-212 |
| 关键词 | LINKED-IMMUNOSORBENT-ASSAY G-QUADRUPLEX APTAMERS SINGLE-STRANDED-DNA MOLECULES SELECTION ENZYME INVITRO LIGANDS BIND |
| ISSN号 | 0956-5663 |
| 通讯作者 | yang xr |
| 中文摘要 | we report an aptasensor for biosensing of ochratoxin a (ota) using aptamer-dnazyme hairpin as biorecognition element. the structure of this engineered nucleic acid includes the horseradish peroxidase (hrp)-mimicking dnazyme and the ota specific aptamer sequences. a blocking tail captures a part of these sequences in the stem region of the hairpin. in the presence of ota, the hairpin is opened due to the formation of the aptamer-analyte complex. as a result, self-assembly of the active hrp-mimicking dnazyme occurs. the activity of this dnazyme is linearly correlated with ota concentration up to 10 nm, showing a limit of detection of 2.5 nm. (c) 2011 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000301011100030 |
| 公开日期 | 2013-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48322]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Yang C,Lates V,Prieto-Simon B,et al. Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A[J]. biosensors & bioelectronics,2012,32(1):208-212. |
| APA | Yang C,Lates V,Prieto-Simon B,Marty JL,&Yang XR.(2012).Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A.biosensors & bioelectronics,32(1),208-212. |
| MLA | Yang C,et al."Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A".biosensors & bioelectronics 32.1(2012):208-212. |
入库方式： OAI收割
来源：长春应用化学研究所
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