Hyperbranched PEI Grafted by Hydrophilic Amino Acid Segment Poly[N-(2-hydroxyethyl)-L-glutamine] as an Efficient Nonviral Gene Carrier
文献类型：期刊论文
| 作者 | Chen J ; Tian HY ; Guo ZP ; Lin L ; Dong X ; Zhu XJ ; Chen XS |
| 刊名 | journal of applied polymer science
 |
| 出版日期 | 2012 |
| 卷号 | 123期号:4页码:2257-2265 |
| 关键词 | GLYCOL) COPOLYMERS DELIVERY-SYSTEMS POLYETHYLENIMINE THERAPY VECTORS PBLG TRANSFECTION |
| ISSN号 | 0021-8995 |
| 通讯作者 | tian hy |
| 中文摘要 | a polyethylenimine-poly(hydroxyethyl glutamine) copolymer (pei-pheg) was designed and synthesized as a gene delivery system. the molecular structure of pei-pheg was characterized using nuclear magnetic resonance. moreover, pei-pheg/pdna complexes were fabricated and characterized by gel retardation assay, particle size analysis, and zeta potential analysis. the transfection efficiency and cytotoxicity of pei-pheg were evaluated using human cervical carcinoma (hela), human embryonic kidney (hek293), and murine colorectal adenocarcinoma (ct26) cells in vitro. the results show that pei-pheg could effectively form positively charged nanosized particles with pdna; the particle size was in a range of 130.2 to 173.0 nm and the zeta potential was in a range of 27.6 to 41.0 mv. pei-pheg exhibited much lower cytotoxicity and higher gene transfection efficiency than pei-25k with different cell lines in vitro. an animal test was also conducted on a lewis lung carcinoma tumor model in c57/bl6 mice by using subcutaneous intratumoral administration. the results show that in vivo transfection efficiency of pei-pheg was improved greatly compared with that of commercial pei-25k. these results demonstrate that pei-pheg can be a potential nonviral vector for gene delivery systems both in vitro and in vivo. (c) 2011 wiley periodicals, inc. j appl polym sci 123: 2257-2265, 2012 |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000297932300044 |
| 公开日期 | 2013-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48327]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Chen J,Tian HY,Guo ZP,et al. Hyperbranched PEI Grafted by Hydrophilic Amino Acid Segment Poly[N-(2-hydroxyethyl)-L-glutamine] as an Efficient Nonviral Gene Carrier[J]. journal of applied polymer science,2012,123(4):2257-2265. |
| APA | Chen J.,Tian HY.,Guo ZP.,Lin L.,Dong X.,...&Chen XS.(2012).Hyperbranched PEI Grafted by Hydrophilic Amino Acid Segment Poly[N-(2-hydroxyethyl)-L-glutamine] as an Efficient Nonviral Gene Carrier.journal of applied polymer science,123(4),2257-2265. |
| MLA | Chen J,et al."Hyperbranched PEI Grafted by Hydrophilic Amino Acid Segment Poly[N-(2-hydroxyethyl)-L-glutamine] as an Efficient Nonviral Gene Carrier".journal of applied polymer science 123.4(2012):2257-2265. |
入库方式： OAI收割
来源：长春应用化学研究所
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