An electrochemical aptasensor for chiral peptide detection using layer-by-layer assembly of polyelectrolyte-methylene blue/polyelectrolyte-graphene multilayer
文献类型：期刊论文
| 作者 | Qin HX ; Liu JY ; Chen CG ; Wang JH ; Wang EK |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2012 |
| 卷号 | 712页码:127-131 |
| 关键词 | MASS-SPECTROMETRY INTERFERON-GAMMA ULTRATHIN FILMS APTAMER BIOSENSOR VASOPRESSIN ELECTRODES PROTEINS MATRICES |
| ISSN号 | 0003-2670 |
| 通讯作者 | wang jh |
| 中文摘要 | here we demonstrate for the first time that by physically adsorbing aptamer onto conductive film assembled via alternate adsorption of graphene/polyelectrolyte and methylene blue/polyelectrolyte, a label-free electrochemical aptasensor with high sensitivity and selectivity for peptide detection is constructed. graphene multilayer derived from layer-by-layer assembly has played significant roles in this sensing strategy: allowing accumulation of methylene blue, facilitating electron transfer and providing much more adsorption site. as compared to previous electrochemical aptasensors, the current sensor based on graphene multilayer alternated with electroactive molecule layer offers extremely high capability for sensitive detection of target without interference of environmental surrounding. this electroactive probe-confined graphene multilayer confers great flexibility to combine with differential pulse voltammetry (dpv) together. in the presence of target d entiomer of arginine vasopressin (d-vp), the binding of peptide to aptamer block the electron transfer process of mb, leading to decreased current peak of dpv. by this way, this electrochemical aptasensor based on electroactive molecule-intercalated graphene multilayer provide highly sensitive and specific detection of d-vp with the lowest detectable concentration of 1 ng ml(-1) and a wide detection range from 1 to 265 ng ml(-1). (c) 2011 elsevier b.v. all rights reserved. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000300764700016 |
| 公开日期 | 2013-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48370]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Qin HX,Liu JY,Chen CG,et al. An electrochemical aptasensor for chiral peptide detection using layer-by-layer assembly of polyelectrolyte-methylene blue/polyelectrolyte-graphene multilayer[J]. analytica chimica acta,2012,712:127-131. |
| APA | Qin HX,Liu JY,Chen CG,Wang JH,&Wang EK.(2012).An electrochemical aptasensor for chiral peptide detection using layer-by-layer assembly of polyelectrolyte-methylene blue/polyelectrolyte-graphene multilayer.analytica chimica acta,712,127-131. |
| MLA | Qin HX,et al."An electrochemical aptasensor for chiral peptide detection using layer-by-layer assembly of polyelectrolyte-methylene blue/polyelectrolyte-graphene multilayer".analytica chimica acta 712(2012):127-131. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。