Visualizing Human Telomerase Activity with Primer-Modified Au Nanoparticles
文献类型：期刊论文
| 作者 | Wang JS ; Wu L ; Ren JS ; Qu XG |
| 刊名 | small
 |
| 出版日期 | 2012 |
| 卷号 | 8期号:2页码:259-264 |
| 关键词 | AMPLIFICATION PROTOCOL ASSAY MODIFIED GOLD NANOPARTICLES G-QUADRUPLEX LIGANDS COLORIMETRIC DETECTION CATIONIC PORPHYRINS OPTICAL ANALYSIS SMALL MOLECULES DNA CANCER INHIBITION |
| ISSN号 | 1613-6810 |
| 通讯作者 | qu xg |
| 中文摘要 | telomerase is over-expressed in over 85% of all known human tumors. this renders the enzyme a valuable biomarker for cancer diagnosis and an important therapeutic target. the most widely used telomeric repeat amplification protocol (trap) assay has been questioned for telomerase detection. it is reported that human telomerase activity can be visualized by using primer-modified au nanoparticles. the working principle is based on the elongated primers conjugated to the gold nanoparticle (aunp) surface, which can fold into a g-quadruplex to protect the aunps from the aggregation. the developed simple and sensitive colorimetric assay can measure telomerase activity down to 1 hela cell mu l-1. more importantly, this assay can be easily extended to high-throughput and automatic format. the aunp-ts method is pcr-free and therefore avoids the amplification-related errors and becomes more reliable to evaluate telomerase activity. this assay has also been used for initial screening of telomerase inhibitors as anticancer drug agents. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000299100500012 |
| 公开日期 | 2013-06-08 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/48375]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang JS,Wu L,Ren JS,et al. Visualizing Human Telomerase Activity with Primer-Modified Au Nanoparticles[J]. small,2012,8(2):259-264. |
| APA | Wang JS,Wu L,Ren JS,&Qu XG.(2012).Visualizing Human Telomerase Activity with Primer-Modified Au Nanoparticles.small,8(2),259-264. |
| MLA | Wang JS,et al."Visualizing Human Telomerase Activity with Primer-Modified Au Nanoparticles".small 8.2(2012):259-264. |
入库方式： OAI收割
来源：长春应用化学研究所
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